SRPK1 is a poor prognostic indicator and a novel potential therapeutic target for human colorectal cancer

被引：14

作者：

Yi, Nan ^{[1
,2
]}

Xiao, Mingbing ^{[1
,2
]}

Jiang, Feng ^{[2
]}

Liu, Zhaoxiu ^{[2
]}

Ni, Wenkai ^{[2
]}

Lu, Cuihua ^{[2
]}

Ni, Runzhou ^{[2
]}

Chen, Weichang ^{[1
]}

机构：

[1] Soochow Univ, Affiliated Hosp 1, Dept Gastroenterol, 188 Shixi Jie, Suzhou 215006, Jiangsu, Peoples R China

[2] Nantong Univ, Affiliated Hosp, Dept Gastroenterol, Nantong 226001, Jiangsu, Peoples R China

来源：

ONCOTARGETS AND THERAPY | 2018年 / 11卷

关键词：

serine/arginine protein kinase 1; colorectal cancer; growth; angiogenesis; apoptosis; PROTEIN; ANGIOGENESIS; MIGRATION; PHOSPHORYLATION; DIAGNOSIS; BREAST; GROWTH;

D O I：

10.2147/OTT.S172541

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: Serine/argininc protein kinase 1 (SRPK1) is a protein kinase that belongs to the serine/arginine-rich domain family of splicing factors which are essential for splice-site selection, especially the modulation for RNA metabolism, localization, and translation. High expression of SRPK1 has been found in numerous human cancers, but its mechanism in colorectal cancer (CRC) is still rarely reported. Purpose: To investigate the expression of SRPK1 in CRC tissues and cells and determine its functions and mechanism in CRC. Methods: The expression of SRPK1 was explored in human CRC patients and cells by immunohistochemistry, real-time quantitative PCR, and Western blot; Cell Counting Kit-8, Transwell, flow cytometry, and tube formation assay were used to investigate the CRC cell viability, migration, apoptosis, and angiogenesis, respectively. Results: SRPK1 was overexpressed in CRC tumor tissues and cells, and correlated with tumor node metastasis stage; inhibition of SRPK1 by si RNA resulted in decreased cell growth and migration, significantly increased apoptosis, and suppressed angiogenesis. Conclusion: SRPK1 can be a prognostic indicator of CRC and may be a therapeutic target for CRC.

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页码：5359 / 5370

页数：12

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