Detection of mecA and ermA genes and simultaneous identification of Staphylococcus aureus using triplex real-time PCR from Malaysian S-aureus strain collections

被引：14

作者：

Shafiei Sabet, Negar ^{[1
]}

Subramaniam, Geetha ^{[1
]}

Navaratnam, Parasakthi ^{[1
]}

Devi Sekaran, Shamala ^{[1
]}

机构：

[1] Univ Malaya, Fac Med, Dept Med Microbiol, Kuala Lumpur 50603, Malaysia

来源：

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | 2007年 / 29卷 / 05期

关键词：

erythromycin; methicillin; real-time PCR; ermA; Staphylococcus aureus; mecA;

D O I：

10.1016/j.ijantimicag.2006.12.017

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A triplex real-time polymerase chain reaction (PCR) assay was used for the simultaneous detection of mecA (methicillin resistance), ermA (erythromycin resistance) and femA (Staphylococcus aureus identification) genes in a single assay. Among 93 clinical S. aureus hospital isolates, there were 48 methicillin-resistant S. aureus (MRSA) and 45 methicillin-sensitive S. aureus (MSSA) isolates. Screening the isolates using the triplex real-time PCR assay, the mecA, ermA and femA genes were detected in all MRSA isolates. The triplex real-time PCR assay was completed within 3 h and is a useful genotypic method for detecting the resistance determinants as well as for the identification of S. aureus isolates. These findings will assist the clinical laboratory in identifying these resistance genes and S. aureus rapidly, thus benefiting patient therapy. This study represents a valuable source of information for researchers to study the local antibiotic resistance pattern, which can increase our knowledge of the antibiotic resistance profile, using real-time PCR technology. (C) 2007 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.

引用

页码：582 / 585

页数：4

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