Separation of some flavonoids using RP-HPLC-NP-TLC off-line coupled system

被引：16
作者：
Hawryl, MA [1 ]
Soczewinski, E [1 ]
机构：
[1] Med Acad, Dept Inorgan & Analyt Chem, PL-20081 Lublin, Poland
来源：
CHROMATOGRAPHIA | 2000年 / 52卷 / 3-4期
关键词：
column liquid chromatography; thin-layer chromatography; coupled chromatographic systems; two-dimensional chromatography; flavonoids;
D O I：
10.1007/BF02490451
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Methods of separating a test mixture of 10 flavonoids were investigated using isocratic RP-HPLC gradient RP-HPLC and a coupled isocratic RP-HPLC - NP-TLC system. Flavonoid fractions partially separated on an RP column were collected, evaporated, applied to a silica TLC plate and developed using 3-step gradient elution with methanol - ethyl acetate mobile phase. Complete separation of the investigated compounds was by application of coupled RP-HPLC and NP-TLC.
引用
收藏
页码：175 / 178
页数：4
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