Separation of some flavonoids using RP-HPLC-NP-TLC off-line coupled system

被引：17

作者：

Hawryl, MA ^{[1
]}

Soczewinski, E ^{[1
]}

机构：

[1] Med Acad, Dept Inorgan & Analyt Chem, PL-20081 Lublin, Poland

来源：

CHROMATOGRAPHIA | 2000年 / 52卷 / 3-4期

关键词：

column liquid chromatography; thin-layer chromatography; coupled chromatographic systems; two-dimensional chromatography; flavonoids;

D O I：

10.1007/BF02490451

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods of separating a test mixture of 10 flavonoids were investigated using isocratic RP-HPLC gradient RP-HPLC and a coupled isocratic RP-HPLC - NP-TLC system. Flavonoid fractions partially separated on an RP column were collected, evaporated, applied to a silica TLC plate and developed using 3-step gradient elution with methanol - ethyl acetate mobile phase. Complete separation of the investigated compounds was by application of coupled RP-HPLC and NP-TLC.

引用

页码：175 / 178

页数：4

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