Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography

被引：61

作者：

Dang, Yuan-Ye ^{[1
]}

Li, Xiao-Cen ^{[1
]}

Zhang, Qing-Wen ^{[1
]}

Li, Shao-Ping ^{[1
]}

Wang, Yi-Tao ^{[1
]}

机构：

[1] Univ Macau, Inst Chinese Med Sci, Taipa, Macau, Peoples R China

来源：

JOURNAL OF SEPARATION SCIENCE | 2010年 / 33卷 / 11期

关键词：

Curcuma wenyujin; High-performance centrifugal partition chromatography; Preparative separation; Volatile components; COUNTER-CURRENT CHROMATOGRAPHY; SUPERCRITICAL-FLUID EXTRACTION; PRESSURIZED LIQUID EXTRACTION; SEPARATION; RHIZOMES; COMPONENTS; PRODUCTS;

D O I：

10.1002/jssc.200900453

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Six volatile compounds, curdione (1), curcumol (2), germacrone (3), curzerene (4), 1,8-cineole (5) and beta-elemene (6), were successfully isolated from the essential oil of Curcuma wenyujin by high-performance centrifugal partition chromatography using a nonaqueous two-phase solvent system consisting of petroleum ether-acetonitrile-acetone (4:3:1 v/v/v). A total of 8 mg of curdione (1), 4 mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), 9 mg of 1,8-cineole (5) and 17 mg of beta-elemene (6) were isolated from the essential oil (300 mg) in 500 min. Their structures were determined by comparison of their retention times and MS data with those of the authentic samples as well as NMR spectroscopic analysis.

引用

页码：1658 / 1664

页数：7

共 31 条

[11]

Ming Q., 2004, CHIN J GERONTOL, V24, P267

[12]

[聂小华 Nie Xiaohua], 2003, [药物生物技术, Pharmaceutical Biotechnology], V10, P152

[13]

Pharmacopoeia Commission of PRC, 2005, PHARM PEOPL REP CHIN

[14] Quantitative determination of eight components in rhizome (Jianghuang) and tuberous root (Yujin) of Curcuma longa using pressurized liquid extraction and gas chromatography-mass spectrometry [J].

Qin, N. Y. ;

Yang, F. Q. ;

Wang, Y. T. ;

Li, S. P. .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2007, 43 (02) :486-492

[15] Separation and Purification of -Cyperone from Cyperus rotundus with Supercritical Fluid Extraction and High-Speed Counter-Current Chromatography [J].

Shi, Xingang ;

Wang, Xiao ;

Wang, Daijie ;

Geng, Yanling ;

Liu, Jianhua .

SEPARATION SCIENCE AND TECHNOLOGY, 2009, 44 (03) :712-721

[16] Role of counter-current chromatography in the modernisation of Chinese herbal medicines [J].

Sutherland, Ian A. ;

Fisher, Derek .

JOURNAL OF CHROMATOGRAPHY A, 2009, 1216 (04) :740-753

[17]

Wang Yan Wang Yan, 2001, Acta Pharmaceutica Sinica, V36, P849

[18]

[夏泉 Xia Quan], 2004, [中国药理学通报, Chinese Pharmacological Bulletin], V20, P357

[19]

XIAO Y, 2007, SHIPIN YU FAJIAO GON, V33, P147

[20]

XIAO Y, 2007, SHIPIN YU FAJIAO GON, V33, P142

← 1 2 3 4 →