Identification of in vivo substrates of the chaperonin GroEL from Bacillus subtilis

被引：13

作者：

Endo, Ayako ^{[1
]}

Kurusu, Yasurou ^{[1
]}

机构：

[1] Ibaraki Univ, Coll Agr, Mol Microbiol Lab, Inashiki, Ibaraki 3000393, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2007年 / 71卷 / 04期

关键词：

GroEL; substrate; Bacillus subtilis;

D O I：

10.1271/bbb.60640

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated GroEL substrates from Bacillus subtilis 168 using the single-ring mutant of B. subtilis GroEL. We identified 28 candidates for GroEL substrates, of which Spo0B, Ald, Eno, SpoIIP, and FbaA were involved in spore formation, and Rnc, Tuf, Eno, Tsf, and FbaA were essential for B. subtilis growth. As observed at the protein level, the amount of SpoIIP interaction with GroEL increased at 3 h after initiation of sporulation.

引用

页码：1073 / 1077

页数：5

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