EFFECTS OF HIGH AMBIENT TEMPERATURE ON FISH SPERM PLASMA MEMBRANE INTEGRITY AND MITOCHONDRIAL ACTIVITY - A FLOW CYTOMETRIC STUDY

被引：2

作者：

Nagy, Szabolcs Tamas ^{[1
]}

Kakasi, Balazs ^{[2
]}

Pal, Laszlo ^{[1
]}

Havasi, Mate ^{[1
,3
]}

Bercsenyi, Miklos ^{[1
]}

Husveth, Ferenc ^{[1
]}

机构：

[1] Univ Pannonia, Dept Anim Sci & Anim Husb, Georgikon Fac, Deak F 16, H-8360 Keszthely, Hungary

[2] Univ Pannonia, Inst Environm Sci, Wartha Vince 1, H-8200 Veszprem, Hungary

[3] Natl Agr Res & Innovat Ctr, Res Inst Fisheries & Aquaculture, Toronto, ON, Canada

来源：

ACTA BIOLOGICA HUNGARICA | 2016年 / 67卷 / 02期

关键词：

Fish sperm; plasma membrane integrity; mitochondrial activity; high temperature; flow cytometry; CLIMATE-CHANGE; STRESS;

D O I：

10.1556/018.67.2016.2.1

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Local extreme climatic conditions occurring as a result of global climate change may interfere with the reproduction of animals. In the present study fish spermatozoa were incubated at different temperatures (20, 25, 30 and 40 degrees C) for 10 and 30 minutes, respectively and plasma membrane integrity and mitochondrial membrane potential changes were evaluated with flow cytometry using SYBR-14/PI and Mitotracker Deep Red FM fluorescent dyes. No significant differences were found in plasma membrane integrity at either incubation temperatures or time points. Mitotracker Deep Red FM histogram profiles indicating mitochondrial activity showed significant (p < 0.001) alterations in all cases of higher (25, 30 and 40 degrees C) temperature treatments as compared to the samples incubated at 20 degrees C. Our results indicate that fish spermatozoa exposed to high temperatures suffer sublethal damage that cannot be detected with conventional, vital staining techniques.

引用

页码：125 / 132

页数：8

共 24 条

[1] Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population: development of diagnostic criteria
Aitken, R. John
De Iuliis, Geoffry N.
Finnie, Jane M.
Hedges, Andrew
McLachlan, Robert I.
[J]. HUMAN REPRODUCTION, 2010, 25 (10) : 2415 - 2426
[2] Barth A. D., 1989, ABNORMAL MORPHOLOGY
[3] Climate change - Evolutionary response to rapid climate change
Bradshaw, William E.
Holzapfel, Christina M.
[J]. SCIENCE, 2006, 312 (5779) : 1477 - 1478
[4] Climate change and seasonal reproduction in mammals
Bronson, F. H.
[J]. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2009, 364 (1534) : 3331 - 3340
[5] Bronson FH., 1989, MAMMALIAN REPROD BIO
[6] COMPARISON OF FREQUENCY-DISTRIBUTIONS IN FLOW-CYTOMETRY
COX, C
REEDER, JE
ROBINSON, RD
SUPPES, SB
WHEELESS, LL
[J]. CYTOMETRY, 1988, 9 (04): : 291 - 298
[7] Cummins J, 2009, SPERM BIOLOGY: AN EVOLUTIONARY PERSPECTIVE, P185, DOI 10.1016/B978-0-12-372568-4.00005-7
[8] GARNER DL, 1994, J ANDROL, V15, P620
[9] Effects of osmolality on inner mitochondrial transmembrane potential and ATP content in spermatozoa recovered from the testes of striped bass (Morone saxatilis)
Guthrie, H. D.
Woods, L. C., III
Long, J. A.
Welch, G. R.
[J]. THERIOGENOLOGY, 2008, 69 (08) : 1007 - 1012
[10] Effects of hypothermic storage on intracellular calcium, reactive oxygen species formation, mitochondrial function, motility, and plasma membrane integrity in striped bass (Morone saxatilis) sperm
Guthrie, H. D.
Welch, G. R.
Theisen, D. D.
Woods, L. C., III
[J]. THERIOGENOLOGY, 2011, 75 (05) : 951 - 961

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