Multiple microarrays of non-adherent cells on a single 3D stimuli-responsive binary polymer-brush pattern

被引：10

作者：

Hou, Jianwen ^{[1
]}

Chen, Runhai ^{[1
]}

Liu, Jingchuan ^{[1
]}

Wang, Haozheng ^{[1
]}

Shi, Qiang ^{[1
]}

Xin, Zhirong ^{[2
]}

Wong, Shing-Chung ^{[3
]}

Yin, Jinghua ^{[1
]}

机构：

[1] Chinese Acad Sci, State Key Lab Polymer Phys & Chem, Changchun Inst Appl Chem, Changchun 130022, Jilin, Peoples R China

[2] Yantai Univ, Dept Polymer, Sch Chem & Chem Engn, Yantai 264005, Peoples R China

[3] Univ Akron, Dept Mech Engn, Akron, OH 44325 USA

来源：

JOURNAL OF MATERIALS CHEMISTRY B | 2018年 / 6卷 / 29期

基金：

中国国家自然科学基金;

关键词：

CARBOHYDRATE-BINDING PROTEINS; BLOOD-CELLS; SURFACE; GLYCOPOLYMER; LITHOGRAPHY; TEMPERATURE; COATINGS; ADHESION; BEHAVIOR; CAPTURE;

D O I：

10.1039/c8tb01441h

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

We have developed a 3D smart binary polymer-brush pattern on the polymer substrate for inducing multiple cell microarrays aided by a lectin and temperature. The binary polymer-brush pattern composed of poly(N-isopropylacrylamide) (PNIPAM) and poly(d-gluconamidoethyl methacrylate) (PGAMA) brushes is fabricated by combining the photolithography technique with a surface-initiated photo-polymerization (SIPP) method. We demonstrate that well-defined binary polymer-brush patterns with high resolution are fabricated using this facile method. The patterned hierarchical PNIPAM brushes exhibit reversible switching to the adhesion of red blood cells (RBCs) induced by thermo-responsiveness. The PGAMA brush domains resist adhesion of RBCs but bind specifically with concanavalin A (Con A), forming a lectin pattern to capture RBCs in a site-specific manner. Therefore, multiple cell microarrays on a single platform are generated with the aid of Con A and temperature. This novel platform composed of a smart binary polymer-brush pattern is versatile and specific, which opens up pathways to potential applications such as microsensors, biochips and bioassays.

引用

页码：4792 / 4798

页数：7