Tissue distribution, subcellular localization, and enzymatic activity analysis of human SIRT5 isoforms

被引：22
作者：
Du, Yipeng [1 ]
Hu, Hao [1 ,2 ]
Hua, Chaoju [1 ,2 ]
Du, Kang [1 ,2 ]
Wei, Taotao [1 ]
机构：
[1] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 503卷 / 02期
基金：
国家重点研发计划; 中国国家自然科学基金;
关键词：
Sirtuins; SIRT5; Protein isoform; Acylase; Malonylation; Succinylation; Acetylation; LYSINE MALONYLATION; INSULIN-SECRETION; PROTEIN; DESUCCINYLATION; DEACYLASE; DEACETYLATES; SIRTUINS;
D O I：
10.1016/j.bbrc.2018.06.073
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
SIRT5 is one of the seven mammalian sirtuins which are NAD(+)-dependent deacylases. In human beings, SIRT5 gene encodes for four SIRT5 protein isoforms, namely SIRT5(iso1), SIR5(iso2), SIRT5(iso3), and SIRT5(iso4). Previous studies have focused mostly on SIRT5(iso1). Characteristics regarding localization, activity and tissue distribution of the other three SIRT5 isoforms remain unclear. In the present study, we characterized these properties of these SIRT5 isoforms. We found that SIRT5(iso1-3) were mitochondria-localized, while SIRT5(iso4) localized mainly in cytoplasm. SIRT5(iso2-4) had little deacylase activity comparing with SIRT5(iso1). Although cDNAs of all SIRT5 isoforms were readily detected in multiply tissues according to EST database, proteins of SIRT5(iso2-4) were seldom observed in human cell lines. Altogether, we dissected the four isoforms of human SIRT5 protein. (C) 2018 Elsevier Inc. All rights reserved.
引用
收藏
页码：763 / 769
页数：7
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