Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

被引：455

作者：

Cossarizza, Andrea ^{[1
]}

Chang, Hyun-Dong ^{[2
]}

Radbruch, Andreas ^{[2
]}

Acs, Andreas ^{[3
]}

Adam, Dieter ^{[4
]}

Adam-Klages, Sabine ^{[14
]}

Agace, William W. ^{[5
,6
]}

Aghaeepour, Nima ^{[357
,358
,359
]}

Akdis, Mubeccel ^{[7
]}

Allez, Matthieu ^{[8
,9
]}

Almeida, Larissa Nogueira ^{[10
]}

Alvisi, Giorgia ^{[11
]}

Anderson, Graham ^{[12
]}

Andrae, Immanuel ^{[13
]}

Annunziato, Francesco ^{[77
]}

Anselmo, Achille ^{[72
]}

Bacher, Petra ^{[4
,15
]}

Baldari, Cosima T. ^{[16
]}

Bari, Sudipto ^{[353
,354
]}

Barnaba, Vincenzo ^{[17
,18
,19
]}

Barros-Martins, Joana ^{[20
]}

Battistini, Luca ^{[21
]}

Bauer, Wolfgang ^{[22
]}

Baumgart, Sabine ^{[2
]}

Baumgarth, Nicole ^{[23
,24
]}

Baumjohann, Dirk ^{[25
]}

Baying, Bianka ^{[26
]}

Bebawy, Mary ^{[27
]}

Becher, Burkhard ^{[150
,298
]}

Beisker, Wolfgang ^{[29
]}

Benes, Vladimir ^{[26
]}

Beyaert, Rudi ^{[31
]}

Blanco, Alfonso ^{[32
]}

Boardman, Dominic A. ^{[33
,34
]}

Bogdan, Christian ^{[35
,36
,37
]}

Borger, Jessica G. ^{[38
]}

Borsellino, Giovanna ^{[39
,40
]}

Boulais, Philip E. ^{[41
,42
]}

Bradford, Jolene A. ^{[43
]}

Brenner, Dirk ^{[44
,45
,46
,360
]}

Brinkman, Ryan R. ^{[48
,373
]}

Brooks, Anna E. S. ^{[49
]}

Busch, Dirk H. ^{[13
,50
,51
]}

Buescher, Martin ^{[52
]}

Bushnell, Timothy P. ^{[53
,54
]}

Calzetti, Federica ^{[55
]}

Cameron, Garth ^{[56
]}

Cammarata, Ilenia ^{[17
]}

Cao, Xuetao ^{[57
]}

Cardell, Susanna L. ^{[58
]}

机构：

[1] Univ Modena & Reggio Emilia, Dept Med & Surg Sci Children & Adults, Sch Med, Modena, Italy

[2] Deutsch Rheuma Forschungszentrum DRFZ, Berlin, Germany

[3] Friedrich Alexander Univ Erlangen Nuremberg, Dept Biol, Nikolaus Fiebiger Ctr Mol Med, Erlangen, Germany

[4] Christian Albrechts Univ Kiel, Inst Immunol, Kiel, Germany

[5] Tech Univ Denmark, Dept Hlth Technol, Mucosal Immunol Grp, Lyngby, Denmark

[6] Lund Univ, Immunol Sect, Lund, Sweden

[7] Univ Zurich, Swiss Inst Allergy & Asthma Res SIAF, Davos, Switzerland

[8] Univ Paris, Inst Rech St Louis, INSERM, U1160, Paris, France

[9] Hop St Louis, AP HP, Dept Gastroenterol, Paris, France

[10] Univ Lubeck, Inst Syst Inflammat Res, Lubeck, Germany

[11] Humanitas Clin & Res Ctr, Lab Translat Immunol, Rozzano, Italy

[12] Univ Birmingham, Birmingham, England

[13] Tech Univ Munich, Inst Med Mikrobiol Immunol & Hyg, Munich, Germany

[14] Univ Klin Schleswig Holstein, Inst Transfus Med, Kiel, Germany

[15] Christian Albrechts Univ Kiel, Inst Klin Mol Biol, Kiel, Germany

[16] Univ Siena, Dept Life Sci, Siena, Italy

[17] Sapienza Univ Roma, Dipartimento Med Interna & Specialita Med, Rome, Italy

[18] Ist Italiano Tecnol, Ctr Life Nano Sci Sapienza, Rome, Italy

[19] Fdn Cenci Bolognetti, Ist Pasteur, Rome, Italy

[20] Hannover Med Sch, Inst Immunol, Hannover, Germany

[21] IRCCS Fdn Santa Lucia, Rome, Italy

[22] Med Univ Vienna, Dept Dermatol, Div Immunol Allergy & Infect Dis, Vienna, Austria

[23] Univ Calif Davis, Ctr Comparat Med, Davis, CA USA

[24] Univ Calif Davis, Dept Pathol Microbiol & Immunol, Davis, CA USA

[25] Ludwig Maximilians Univ Munchen, Inst Immunol, Biomed Ctr, Fac Med, Planegg Martinsried, Germany

[26] EMBL, Genom Core Facil, Heidelberg, Germany

[27] Univ Technol Sydney, Discipline Pharm, Grad Sch Hlth, Sydney, NSW, Australia

[28] Univ Calif Los Angeles, Dept Bioengn, Los Angeles, CA USA

[29] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Mol Toxicol & Pharmacol, Flow Cytometry Lab, Munich, Germany

[30] Univ Milan, Dept Med Biotechnol & Translat Med, Milan, Italy

[31] Ghent Univ VIB, Dept Biomed Mol Biol, Ctr Inflammat Res, Ghent, Belgium

[32] Univ Coll Dublin, Flow Cytometry Core Technol, UCD Conway Inst, Dublin, Ireland

[33] Univ British Columbia, Dept Surg, Vancouver, BC, Canada

[34] BC Childrens Hosp Res Inst, Vancouver, BC, Canada

[35] Univ Klinikum Erlangen, Mikrobiol Inst, Klin Mikrobiol Immunol & Hyg, Erlangen, Germany

[36] Friedrich Alexander Univ FAU Erlangen Nurnberg, Erlangen, Germany

[37] Med Immunol Campus Erlangen, Erlangen, Germany

[38] Monash Univ, Dept Pathol & Immunol, Melbourne, Vic, Australia

[39] Fdn Santa Lucia IRCCS, Neuroimmunol Unit, Rome, Italy

[40] Fdn Santa Lucia IRCCS, Flow Cytometry Unit, Rome, Italy

[41] Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY USA

[42] Ruth L & David S Gottesman Inst Stem Cell & Regen, Bronx, NY USA

[43] Thermo Fisher Sci, Eugene, OR USA

[44] Luxembourg Inst Hlth, Dept Infect & Immun Expt & Mol Immunol, Esch Sur Alzette, Luxembourg

[45] Univ Southern Denmark, Odense Univ Hosp, Dept Dermatol, Odense Res Ctr Anaphylaxis, Odense, Denmark

[46] Univ Southern Denmark, Odense Univ Hosp, Allergy Ctr, Odense Res Ctr Anaphylaxis, Odense, Denmark

[47] BD Life Sci, Ashland, OR USA

[48] Univ British Columbia, Dept Med Genet, Vancouver, BC, Canada

[49] Univ Auckland, Sch Biol Sci, Maurice Wilkins Ctr, Auckland, New Zealand

[50] German Ctr Infect Res DZIF, Munich, Germany

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 2019年 / 49卷 / 10期

基金：

欧洲研究理事会;

关键词：

D O I：

10.1002/eji.201970107

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.

引用

页码：1457 / 1973

页数：517

共 206 条

[1]

Cossarizza A., Chang H.-D., Radbruch A., Akdis M., Andra I., Annunziato F., Bacher P., Et al., Guidelines for the use of flow cytometry and cell sorting in immunological studies, Eur. J. Immunol., 47, pp. 1584-1797, (2017)

[2]

Mack J., Fulwyler Particle Separator

[3]

Kachel V., Fellner-Feldegg H., Menke E., Hydrodynamic properties of flow cytometry instruments, Flow cytometry and sorting, pp. 27-44, (1990)

[4]

Crosland-Taylor P.J., A device for counting small particles suspended in a fluid through a tube, Nature, 171, pp. 37-38, (1953)

[5]

Gucker F.T., O'Konski C.T., Pickard H.B., Pitts J.N., A photoelectronic counter for colloidal particles, J. Am. Chem. Soc., 69, pp. 2422-2431, (1947)

[6]

Van den Engh G.J., Flow cytometer droplet formation system. US patent US 6861265 B1

[7]

Van den Engh G.J., Particle separating apparatus and method. US patent US 5819948 A

[8]

Norton P.O., Vane D.R., Javadi S., Fixed mounted sorting cuvette with user replaceable nozzle

[9]

Dolezel J., Gohde W., Sex determination in dioecious plants Melandrium album and M. rubrum using high-resolution flow cytometry, Cytometry, 19, pp. 103-106, (1995)

[10]

Steen H.B., Lindau T., Sorensen O., A simple, high resolution flow cytometer based on a standard fluorescence microscope, Proceedings of the IVth international symposium on flow cytometry (pulse cytophotometry), pp. 31-33, (1980)

← 1 2 3 4 5 6 7 8 9 10 →