Tissue adhesive, rapid forming, and sprayable ECM hydrogel via recombinant tyrosinase crosslinking

被引：114

作者：

Kim, Su-Hwan ^{[1
,2
]}

Lee, Sang-Hyuk ^{[1
,2
,3
]}

Lee, Ju-Eun ^{[1
,2
,3
]}

Park, Sung Jun ^{[2
]}

Kim, Kyungmin ^{[1
,2
]}

Kim, In Seon ^{[2
]}

Lee, Yoon-Sik ^{[1
,2
]}

Hwang, Nathaniel S. ^{[1
,2
,4
]}

Kim, Byung-Gee ^{[1
,2
,3
]}

机构：

[1] Seoul Natl Univ, Interdisciplinary Program Bioengn, Seoul 151742, South Korea

[2] Seoul Natl Univ, Sch Chem & Biol Engn, Seoul 151742, South Korea

[3] Seoul Natl Univ, Inst Mol Biol & Genet, Inst Bioengn, Seoul 151742, South Korea

[4] Seoul Natl Univ, Inst Chem Proc, Seoul 151742, South Korea

来源：

BIOMATERIALS | 2018年 / 178卷

关键词：

Tyrosinase; ECM hydrogel; Tissue adhesive; Injectable hydrogel; Sprayable hydrogel; HYALURONIC-ACID HYDROGELS; STREPTOMYCES-AVERMITILIS MA4680; CHITOSAN; HYDROXYLATION; BIOMATERIALS; RESVERATROL; DEGRADATION; INHIBITION; CHEMISTRY; CATECHIN;

D O I：

10.1016/j.biomaterials.2018.04.057

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We report on a tissue adhesive hydrogel based on novel recombinant tyrosinase mediated crosslinking. The adhesive hydrogels were fabricated by the site-directed coupling of tyramine-conjugated hyaluronic acid (HA_t, 1% wiv) and gelatin (3% wiv) (HG_gel) with novel tyrosinase derived from Streptomyces avermitilis (SA_Ty). The enzyme-based crosslinking by SA_Ty was fast, with less than 50s for complete gelation, and the SA_Ty based crosslinking enhanced the physical properties and adhesive strength of the hydrogel significantly with the native tissue samples. Furthermore, by optimizing the injection conditions, we tailored the enzyme-based crosslinking hydrogels to be injectable and sprayable with a medical syringe and commercial airbrush nozzle, respectively. An in vivo analysis of the adhesive hydrogel showed a negligible immune reaction. In this study, demonstrate that the novel enzyme-based cross linking hydrogel has a robust potential in tissue engineering and regenerative medicine. (C) 2018 Elsevier Ltd. All rights reserved.

引用

页码：401 / 412

页数：12

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