Tissue adhesive, rapid forming, and sprayable ECM hydrogel via recombinant tyrosinase crosslinking

被引：111
作者：
Kim, Su-Hwan [1 ,2 ]
Lee, Sang-Hyuk [1 ,2 ,3 ]
Lee, Ju-Eun [1 ,2 ,3 ]
Park, Sung Jun [2 ]
Kim, Kyungmin [1 ,2 ]
Kim, In Seon [2 ]
Lee, Yoon-Sik [1 ,2 ]
Hwang, Nathaniel S. [1 ,2 ,4 ]
Kim, Byung-Gee [1 ,2 ,3 ]
机构：
[1] Seoul Natl Univ, Interdisciplinary Program Bioengn, Seoul 151742, South Korea
[2] Seoul Natl Univ, Sch Chem & Biol Engn, Seoul 151742, South Korea
[3] Seoul Natl Univ, Inst Mol Biol & Genet, Inst Bioengn, Seoul 151742, South Korea
[4] Seoul Natl Univ, Inst Chem Proc, Seoul 151742, South Korea
来源：
BIOMATERIALS | 2018年 / 178卷
关键词：
Tyrosinase; ECM hydrogel; Tissue adhesive; Injectable hydrogel; Sprayable hydrogel; HYALURONIC-ACID HYDROGELS; STREPTOMYCES-AVERMITILIS MA4680; CHITOSAN; HYDROXYLATION; BIOMATERIALS; RESVERATROL; DEGRADATION; INHIBITION; CHEMISTRY; CATECHIN;
D O I：
10.1016/j.biomaterials.2018.04.057
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
We report on a tissue adhesive hydrogel based on novel recombinant tyrosinase mediated crosslinking. The adhesive hydrogels were fabricated by the site-directed coupling of tyramine-conjugated hyaluronic acid (HA_t, 1% wiv) and gelatin (3% wiv) (HG_gel) with novel tyrosinase derived from Streptomyces avermitilis (SA_Ty). The enzyme-based crosslinking by SA_Ty was fast, with less than 50s for complete gelation, and the SA_Ty based crosslinking enhanced the physical properties and adhesive strength of the hydrogel significantly with the native tissue samples. Furthermore, by optimizing the injection conditions, we tailored the enzyme-based crosslinking hydrogels to be injectable and sprayable with a medical syringe and commercial airbrush nozzle, respectively. An in vivo analysis of the adhesive hydrogel showed a negligible immune reaction. In this study, demonstrate that the novel enzyme-based cross linking hydrogel has a robust potential in tissue engineering and regenerative medicine. (C) 2018 Elsevier Ltd. All rights reserved.
引用
收藏
页码：401 / 412
页数：12
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