Expression of the HSV-1 (F) glycoprotein B gene in insect cells infected by HcNPV recombinant

被引：0

作者：

Cha, SC ^{[1
]}

Kang, H ^{[1
]}

Lee, SY ^{[1
]}

Park, GJ ^{[1
]}

Lee, HH ^{[1
]}

机构：

[1] Konkuk Univ, Dept Biol, Seoul 143701, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2000年 / 10卷 / 03期

关键词：

HSV-1; glycoprotein B; baculovirus; neutralizing antibody;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) gene in the pHLA-21 plasmid was inserted into a baculovirus (Hyphantria cunea nuclear polyhedrosis virus) expression vector (lacZ-HcNPV) to construct a recombinant virus gB-HcNPV expressing gB. Spodoptera frugiperda cells infected with this recombinant virus synthesized and processed gB of approximately 120 kDa, which cross-reacted with the monoclonal antibody to gB. The recombinant gB was identified on the membrane of the insect cells using an immunofluorescence assay. Antibodies to this recombinant raised in mice recognized the viral gB and neutralized the infectivity of the HSV-1 in vitro. These results show that the gB gene has the potential to be expressed in insect cells. They also demonstrate that it is possible to produce a mature protein by gene transfer in eukaryotic cells, and indicate the utility of the lacZ-HcNPV-insect cell system for producing and characterizing edkaryotic proteins. Furthermore, the neutralizing antibodies would appear to protect mice against HSV. Accordingly, this particular recombinant protein may be useful in the development of a subunit vaccine.

引用

页码：355 / 362

页数：8

共 53 条

[1] THE UL10 GENE OF HERPES-SIMPLEX VIRUS-1 ENCODES A NOVEL VIRAL GLYCOPROTEIN, GM, WHICH IS PRESENT IN THE VIRION AND IN THE PLASMA-MEMBRANE OF INFECTED-CELLS [J].