Heat shock factor 1 is required for constitutive Hsp70 expression and normal lens development in embryonic zebrafish

被引:23
作者
Evans, Tyler G. [1 ]
Belak, Zachery [1 ]
Ovsenek, Nick [1 ]
Krone, Patrick H. [1 ]
机构
[1] Univ Saskatchewan, Dept Anat & Cell Biol, Saskatoon, SK S7N 5E5, Canada
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY | 2007年 / 146卷 / 01期
基金
加拿大自然科学与工程研究理事会;
关键词
zebrafish embryo; heat shock protein 70; heat shock factor; lens development; morpholino;
D O I
10.1016/j.cbpa.2006.09.023
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Heat shock factors (HSFs) are the major transcription factors responsible for heat-induced upregulation of heat shock protein (Hsp) genes. All three mammalian HSFs (HSF1, HSF2, HSF4) have also been shown to be required for normal mammalian development. It is currently unknown if HSFs play similarly important roles during normal development of non-mammalian vertebrates. In the present study, a morpholino modified antisense oligonucleotide (MO) approach targeted against hsf1 mRNA (hsf1-MO) was used to examine the requirement of HSF1 in zebrafish development. Embryos depleted of HSF1 displayed a reproducible small eye phenotype characterized by an immature lens and a disorganized retinal structure. These defects were strikingly similar to those observed when constitutive, lens specific Hsp70 expression was reduced through the microinjection of MO targeting hsp70. The data suggest that HSF1 is involved in regulating constitutive lens specific expression of hsp70 in the embryonic zebrafish. This conclusion is supported by a marked reduction in Hsp70 protein in hsf1-MO injected embryos. Microinjection of MO targeted to hsf2 mRNA (hsf2-MO) did not result in a small eye phenotype in a significant number of embryos. These data also suggest that HSF1 and HSF2 play distinct roles in non-mammalian vertebrates, similarly to what has been demonstrated previously in mouse. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:131 / 140
页数:10
相关论文
共 45 条
[11]   Expression of Hsp27 in retinal ganglion cells of the rat during postnatal development [J].
Hawkes, EL ;
Krueger-Naug, AMR ;
Nickerson, PEB ;
Myers, TL ;
Currie, RW ;
Clarke, DB .
JOURNAL OF COMPARATIVE NEUROLOGY, 2004, 478 (02) :143-148
[12]   Morpholino oligos: Making sense of antisense? [J].
Heasman, J .
DEVELOPMENTAL BIOLOGY, 2002, 243 (02) :209-214
[13]   SIMPLE METHYLENE BLUE-AZURE II-BASIC FUCHSIN STAIN FOR EPOXY-EMBEDDED TISSUE SECTIONS [J].
HUMPHREY, CD ;
PITTMAN, FE .
STAIN TECHNOLOGY, 1974, 49 (01) :9-14
[14]   Impaired IgG production in mice deficient for heat shock transcription factor 1 [J].
Inouye, S ;
Izu, H ;
Takaki, E ;
Suzuki, H ;
Shirai, M ;
Yokota, Y ;
Ichikawa, H ;
Fujimoto, M ;
Nakai, A .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (37) :38701-38709
[15]   Multiple functions of Drosophila heat shock transcription factor in vivo [J].
Jedlicka, P ;
Mortin, MA ;
Wu, C .
EMBO JOURNAL, 1997, 16 (09) :2452-2462
[16]   Brain abnormalities, defective meiotic chromosome synapsis and female subfertility in HSF2 null mice [J].
Kallio, M ;
Chang, YH ;
Manuel, M ;
Alastalo, TP ;
Rallu, M ;
Gitton, Y ;
Pirkkala, L ;
Loones, MT ;
Paslaru, L ;
Larney, S ;
Hiard, S ;
Morange, M ;
Sistonen, L ;
Mezger, V .
EMBO JOURNAL, 2002, 21 (11) :2591-2601
[17]   SELECTION OF NEW HSF1 AND HSF2 DNA-BINDING SITES REVEALS DIFFERENCES IN TRIMER COOPERATIVITY [J].
KROEGER, PE ;
MORIMOTO, RI .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (11) :7592-7603
[18]   Heat shock gene expression and function during zebrafish embryogenesis [J].
Krone, PH ;
Evans, TG ;
Blechinger, SR .
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2003, 14 (05) :267-274
[19]   Suppression of lens growth by αA-crystallin promoter-driven expression of diphtheria toxin results in disruption of retinal cell organization in zebrafish [J].
Kurita, R ;
Sagara, H ;
Aoki, Y ;
Link, BA ;
Arai, K ;
Watanabe, S .
DEVELOPMENTAL BIOLOGY, 2003, 255 (01) :113-127
[20]   Disruption of zebrafish somite development by pharmacologic inhibition of Hsp90 [J].
Lele, Z ;
Hartson, SD ;
Martin, CC ;
Whitesell, L ;
Matts, RL ;
Krone, PH .
DEVELOPMENTAL BIOLOGY, 1999, 210 (01) :56-70