Visual and modular detection of pathogen nucleic acids with enzyme-DNA molecular complexes

被引:78
作者
Ho, Nicholas R. Y. [1 ,2 ]
Lim, Geok Soon [1 ,2 ]
Sundah, Noah R. [1 ,3 ]
Lim, Diana [4 ]
Loh, Tze Ping [1 ,5 ]
Shao, Huilin [1 ,2 ,3 ,6 ]
机构
[1] Natl Univ Singapore, Biomed Inst Global Hlth Res & Technol, Singapore 117599, Singapore
[2] Agcy Sci Technol & Res, Inst Mol & Cell Biol, Singapore 138673, Singapore
[3] Natl Univ Singapore, Dept Biomed Engn, Fac Engn, Singapore 117583, Singapore
[4] Natl Univ Singapore Hosp, Dept Pathol, Singapore 119074, Singapore
[5] Natl Univ Singapore Hosp, Dept Lab Med, Singapore 117599, Singapore
[6] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Surg, Singapore 119228, Singapore
来源
NATURE COMMUNICATIONS | 2018年 / 9卷
关键词
CARCINOMA CELL-LINES; HUMAN-PAPILLOMAVIRUS; ISOTHERMAL AMPLIFICATION; CERVICAL-CANCER; ZIKA VIRUS; SEQUENCES; POINT; PCR;
D O I
10.1038/s41467-018-05733-0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Rapid, visual detection of pathogen nucleic acids has broad applications in infection management. Here we present a modular detection platform, termed enzyme-assisted nanocomplexes for visual identification of nucleic acids (enVision). The system consists of an integrated circuit of enzyme-DNA nanostructures, which function as independent recognition and signaling elements, for direct and versatile detection of pathogen nucleic acids from infected cells. The built-in enzymatic cascades produce a rapid color readout for the naked eye; the assay is thus fast (<2 h), sensitive (<10 amol), and readily quantified with smart-phones. When implemented on a configurable microfluidic platform, the technology demonstrates superior programmability to perform versatile computations, for detecting diverse pathogen targets and their virus-host genome integration loci. We further design the enVision platform for molecular-typing of infections in patient endocervical samples. The technology not only improves the clinical inter-subtype differentiation, but also expands the intra-subtype coverage to identify previously undetectable infections.
引用
收藏
页数:11
相关论文
共 38 条
[21]   Detection of Nucleic Acids of the Fish Pathogen Yersinia ruckeri from Planktonic and Biofilm Samples with a CRISPR/Cas13a-Based Assay [J].
Calderon, Ivan L. ;
Barros, M. Jose ;
Fernandez-Navarro, Nicolas ;
Acuna, Lillian G. .
MICROORGANISMS, 2024, 12 (02)
[22]   Dual Signal Amplification by eATRP and DNA-Templated Silver Nanoparticles for Ultrasensitive Electrochemical Detection of Nucleic Acids [J].
Sun, Haobo ;
Kong, Jinming ;
Wang, Qiangwei ;
Liu, Qianrui ;
Zhang, Xueji .
ACS APPLIED MATERIALS & INTERFACES, 2019, 11 (31) :27568-27573
[23]   Molecular beacon-based enzyme-free strategy for amplified DNA detection [J].
Huang, Jiahao ;
Wu, Jueqi ;
Li, Zhigang .
BIOSENSORS & BIOELECTRONICS, 2016, 79 :758-762
[24]   A fully integrated paperfluidic molecular diagnostic chip for the extraction, amplification, and detection of nucleic acids from clinical samples [J].
Rodriguez, Natalia M. ;
Wong, Winnie S. ;
Liu, Lena ;
Dewar, Rajan ;
Klapperich, Catherine M. .
LAB ON A CHIP, 2016, 16 (04) :753-763
[25]   Multiplex Detection of Nucleic Acids Using Recombinase Polymerase Amplification and a Molecular Colorimetric 7-Segment Display [J].
Li, Jia ;
Pollak, Nina M. ;
Macdonald, Joanne .
ACS OMEGA, 2019, 4 (07) :11388-11396
[26]   DNAzyme Molecular Beacon Probes for Target-Induced Signal-Amplifying Colorimetric Detection of Nucleic Acids [J].
Fu, Rongzhan ;
Li, Taihua ;
Lee, Soo Suk ;
Park, Hyun Gyu .
ANALYTICAL CHEMISTRY, 2011, 83 (02) :494-500
[27]   Dual Protein Corona-Mediated Target Recognition System for Visual Detection and Single-Molecule Counting of Nucleic Acids [J].
Zhang, Baoshui ;
Zhang, Pengbo ;
Wang, Hao ;
Wang, Xiaoyu ;
Hu, Zhian ;
Wang, Fangfang ;
Li, Zhengping .
ACS NANO, 2025, 19 (07) :6929-6941
[28]   Stoichiometric Tuning of PNA Probes to Au0.8Ag0.2 Alloy Nanoparticles for Visual Detection of Nucleic Acids in Plasma [J].
Goyal, Garima ;
Ammanath, Gopal ;
Palaniappan, Alagappan ;
Liedberg, Bo .
ACS SENSORS, 2020, 5 (08) :2476-2485
[29]   Nucleotide base analog pyrrolo-deoxycytidine as fluorescent probe signal for enzyme-free and signal amplified nucleic acids detection [J].
Duan, Zhijuan ;
Li, Zhuo ;
Dai, Jianyuan ;
He, Hongfei ;
Xiao, Dan .
TALANTA, 2017, 164 :34-38
[30]   Detection of trace amounts of target DNA from massive background of nucleic acids by using the LM-PCR-based preamplification method [J].
Pan, Xiaoming ;
Wang, Jing ;
Zhang, Yanfang ;
Dong, Ping ;
Li, Chunchuan ;
Liang, Xingguo .
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2017, 64 (06) :879-887