Improved method for EPR detection of DEPMPO-superoxide radicals by liquid nitrogen freezing

被引：20
|
作者：
Dambrova, M
Baumane, L
Kalvinsh, I
Wikberg, JES
机构：
[1] Latvian Inst Organ Synth, Dept Med Chem, LV-1006 Riga, Latvia
[2] Uppsala Univ, Dept Pharmaceut Biosci, Div Pharmacol, Uppsala, Sweden
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 275卷 / 03期
关键词：
DEPMPO; xanthine oxidase; superoxide; EPR; superoxide dismutase; allopurinol; liquid nitrogene;
D O I：
10.1006/bbrc.2000.3387
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
5-Diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO) is frequently used as a spin trap for the measurement of superoxide by EPR spectrometry. However, its half life is fairly short in room temperature. We here show that superoxide radicals trapped by DEPMPO can be successfully recorded at -196 degrees C. Moreover, we show that the signal intensity remains unaltered for up to 7 days, when the samples are stored in liquid nitrogen. Our new approach for measurement of superoxide should greatly simplify the studies of this important radical in biological systems. (C) 2000 Academic Press.
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页码：895 / 898
页数：4
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