Rapid Multilabel Detection of Geranylgeranylated Proteins by Using Bioorthogonal Ligation Chemistry

被引：39
|
作者：
Berry, Alexandra F. H. [1 ,2 ]
Heal, William P. [1 ,2 ]
Tarafder, Abul K. [2 ,3 ]
Tolmachova, Tanya [2 ,3 ]
Baron, Rudi A. [2 ,3 ]
Seabra, Miguel C. [2 ,3 ]
Tate, Edward W. [1 ,2 ]
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England
[2] Univ London Imperial Coll Sci Technol & Med, Chem Biol Ctr, London SW7 2AZ, England
[3] Univ London Imperial Coll Sci Technol & Med, Natl Heart & Lung Inst, London SW7 2AZ, England
来源：
CHEMBIOCHEM | 2010年 / 11卷 / 06期
基金：
英国生物技术与生命科学研究理事会; 英国惠康基金;
关键词：
bioorthogonal ligation; click chemistry; geranylgeranylation; post-translational modifications; proteomics; transferases; N-MYRISTOYL TRANSFERASE; MAMMALIAN-CELLS; IN-VIVO; FARNESYLTRANSFERASE; MECHANISM; AZIDES;
D O I：
10.1002/cbic.201000087
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
(Figure Presented) One tag, two: Defects in protein prenylation lead to a number of serious diseases; here we show that an azide-tagged substrate enables multilabel de-tection of defective protein geranylatlon by two geranylgeranyl transfer-ases. Rapid in-gel visualization and affin-ity purification have been demonstrated on recombinant proteins, cell lines, and mammalian disease models. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：771 / 773
页数：3
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