Rapid Multilabel Detection of Geranylgeranylated Proteins by Using Bioorthogonal Ligation Chemistry

被引：39

作者：

Berry, Alexandra F. H. ^{[1
,2
]}

Heal, William P. ^{[1
,2
]}

Tarafder, Abul K. ^{[2
,3
]}

Tolmachova, Tanya ^{[2
,3
]}

Baron, Rudi A. ^{[2
,3
]}

Seabra, Miguel C. ^{[2
,3
]}

Tate, Edward W. ^{[1
,2
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England

[2] Univ London Imperial Coll Sci Technol & Med, Chem Biol Ctr, London SW7 2AZ, England

[3] Univ London Imperial Coll Sci Technol & Med, Natl Heart & Lung Inst, London SW7 2AZ, England

来源：

CHEMBIOCHEM | 2010年 / 11卷 / 06期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

bioorthogonal ligation; click chemistry; geranylgeranylation; post-translational modifications; proteomics; transferases; N-MYRISTOYL TRANSFERASE; MAMMALIAN-CELLS; IN-VIVO; FARNESYLTRANSFERASE; MECHANISM; AZIDES;

D O I：

10.1002/cbic.201000087

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Figure Presented) One tag, two: Defects in protein prenylation lead to a number of serious diseases; here we show that an azide-tagged substrate enables multilabel de-tection of defective protein geranylatlon by two geranylgeranyl transfer-ases. Rapid in-gel visualization and affin-ity purification have been demonstrated on recombinant proteins, cell lines, and mammalian disease models. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：771 / 773

页数：3

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