Analysis of bacterial chemotactic response using dynamic laser speckle

被引：53

作者：

Murialdo, Silvia E. ^{[2
]}

Sendra, Gonzalo H. ^{[1
]}

Passoni, Lucia I. ^{[4
]}

Arizaga, Ricardo ^{[3
]}

Froilan Gonzalez, J. ^{[2
]}

Rabal, Hector ^{[3
]}

Trivi, Marcelo ^{[3
]}

机构：

[1] Univ Nacl Mar del Plata, Fac Ingn, Lab Laser, RA-7600 Mar Del Plata, Buenos Aires, Argentina

[2] Univ Nacl Mar del Plata, Fac Ingn, Dept Quim, Grp Ingn Bioquim, RA-7600 Mar Del Plata, Buenos Aires, Argentina

[3] Univ Nacl Mar del Plata, Fac Ingn, UID Optimo, Ctr Invest Opt CONICET La Plata CIC, RA-1897 La Plata, Buenos Aires, Argentina

[4] Univ Nacl Mar del Plata, Fac Ingn, Lab Bioingn, RA-7600 Mar Del Plata, Buenos Aires, Argentina

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2009年 / 14卷 / 06期

关键词：

dynamic speckle; chemotaxis; bacteria; biospeckle; MOTILITY; DEGRADATION;

D O I：

10.1117/1.3262608

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chemotaxis has a meaningful role in several fields, such as microbial physiology, medicine and biotechnology. We present a new application of dynamic laser speckle (or biospeckle) to detect different degrees of bacterial motility during chemotactic response experiments. Encouraging results showed different bacterial dynamic responses due to differences in the hardness of the support in the swarming plates. We compare this method to a conventional technique that uses white light. Both methods showed to be analogous and, in some cases, complementary. The results suggest that biospeckle processed images can be used as an alternative method to evaluate bacterial chemotactic response and can supply additional information about the bacterial motility in different areas of the swarm plate assay that might be useful for biological analysis. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3262608]

引用

页数：6

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