A new experimental culture medium for cultivation of Leishmania amazonensis: its efficacy for the continuous in vitro growth and differentiation of infective promastigote forms

被引：25

作者：

Rodrigues, Igor de Almeida ^{[1
,2
]}

da Silva, Bianca Alcantara ^{[1
]}

Souza dos Santos, Andre Luis ^{[1
]}

Vermelho, Alane Beatriz ^{[1
]}

Alviano, Celuta Sales ^{[1
]}

Santos Rosa, Maria do Socorro ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, CCS, IMPPG, Dept Microbiol Geral, BR-21941902 Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, Ctr Tecnol, Inst Quim, BR-21941902 Rio De Janeiro, Brazil

来源：

PARASITOLOGY RESEARCH | 2010年 / 106卷 / 05期

关键词：

AMASTIGOTE-LIKE FORMS; AXENIC AMASTIGOTES; LARGE NUMBERS; PARASITES; DONOVANI;

D O I：

10.1007/s00436-010-1775-4

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Parasites from the genus Leishmania cause a variety of disease states in humans and other mammals in tropical and subtropical regions, which include cutaneous, mucocutaneous and visceral leishmaniasis. The elaboration of a culture medium for the in vitro cultivation of Leishmania spp., which promotes the growth and differentiation of the parasites, is an important tool for diagnosis, biochemical, biological and immunological studies in the genus. Herein, we have reported the development of a rapid, inexpensive and reliable monophasic culture medium. The novel medium, designated PBHIL, promoted an excellent parasite growth, generating high quantities of promastigotes with long-term viability, and was able to induce cellular differentiation of L. amazonensis promastigotes to the amastigote-like forms (93%). Additionally, we reported the influence of this novel medium on the biochemical characteristics of L. amazonensis and on the interaction of this parasite parasites with mammalian macrophages.

引用

页码：1249 / 1252

页数：4

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