Thermostable glycerol kinase from Thermus flavus:: cloning, sequencing, and expression of the enzyme gene

被引：11

作者：

Huang, HS

Kabashima, T

Ito, K

Yin, CH

Nishiya, Y

Kawamura, Y

Yoshimoto, T

机构：

[1] Nagasaki Univ, Sch Pharmaceut Sci, Nagasaki 852, Japan

[2] Toyobo Tsuruga Inst Biotechnol, Fukui 914, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1998年 / 1382卷 / 02期

关键词：

glycerol kinase; thermostable enzyme; gene cloning; nucleotide sequence; (Thermus flavus);

D O I：

10.1016/S0167-4838(97)00206-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The thermostable glycerol kinase (EC 2.7.1.30) gene from Thermus flavus was cloned and expressed in Escherichia toll DH5 alpha. An open reading frame of 1488 bp for the glycerol kinase gene (glpK) starting with an ATG methionine codon was found, which encodes a protein of 496 amino acid residues whose calculated molecular weight is 54,835. The amino acid sequence of T. flavus glycerol kinase is 80.6% and 64.1% identical with those of Bacillus subtilis and E. coli. Transformants of E. coli DH5 alpha harboring plasmid pGYK12 with a 1505 bp chromosomal DNA fragment containing the T. flavus glycerol kinase gene showed about 23.8-fold higher glycerol kinase activity than T. flavus. (C) 1998 Elsevier Science B.V.

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收藏

页码：186 / 190

页数：5

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