The interaction of sonochemically synthesized gold nanoparticles with serum albumins

被引：91

作者：

Naveenraj, Selvaraj ^{[1
]}

Anandan, Sambandam ^{[1
]}

Kathiravan, Arunkumar ^{[2
]}

Renganathan, Rajalingam ^{[2
]}

Ashokkumar, Muthupandian ^{[3
]}

机构：

[1] Natl Inst Technol, Nanomat & Solar Energy Convers Lab, Dept Chem, Tiruchirappalli 620015, Tamil Nadu, India

[2] Bharathidasan Univ, Sch Chem, Tiruchirappalli 620024, India

[3] Univ Melbourne, Sch Chem, Melbourne, Vic 3010, Australia

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2010年 / 53卷 / 03期

关键词：

Sonochemical reduction; Gold nanoparticles; Serum albumins; Fluorescence quenching; COLLOIDAL GOLD; SIZE; AG;

D O I：

10.1016/j.jpba.2010.03.039

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Spherical gold nanoparticles of approximately 16 nm were synthesized using a sonochemical reduction method and characterized using UV-vis spectroscopy, atomic force microscopy (AFM) and transmission electron microscopy (TEM) The binding of these gold nanoparticles with bovine serum albumin (BSA) and human serum albumin (HSA) was investigated using UV-vis absorption and fluorescence spectroscopic techniques. A strong quenching of the fluorescence from serum albumins was observed due to the formation of a ground state complex with gold nanoparticles (static quenching) The fluorescence quenching constants, number of binding sites and binding constants were determined using Stern-Volmer and Benesi-Hildebrand plots. Using Forster Resonance Energy Transfer (FRET) theory, the distance between the donor (serum albumins) and acceptor (gold nanoparticles) was obtained, which showed that HSA has more affinity towards sonochemically synthesized gold nanoparticles compared to gold nanoparticles synthesized using other methods (C) 2010 Elsevier B V All rights reserved.

引用

页码：804 / 810

页数：7

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