Tobacco Etch Virus protease: A shortcut across biotechnologies

被引：53
作者：
Cesaratto, Francesca [1 ]
Burrone, Oscar R. [1 ]
Petris, Gianluca [2 ]
机构：
[1] ICGEB, Trieste, Italy
[2] Univ Trento, CIBIO, Trento, Italy
来源：
JOURNAL OF BIOTECHNOLOGY | 2016年 / 231卷
关键词：
TEV protease; Self-cleavage; In vivo applications; Protein engineering; Split-TEV; Subcellular targeting; TANDEM AFFINITY PURIFICATION; TEV PROTEASE; IN-VIVO; ESCHERICHIA-COLI; FUSION PROTEINS; CLEAVAGE SITE; SUBSTRATE-SPECIFICITY; RECOMBINANT PROTEINS; MUTATIONAL ANALYSIS; WILD-TYPE;
D O I：
10.1016/j.jbiotec.2016.06.012
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
About thirty years ago, studies on the RNA genome of Tobacco Etch Virus revealed the presence of an efficient and specific protease, called Tobacco Etch Virus protease (TEVp), that was part of the Nuclear Inclusion a (NIa) enzyme. TEVp is an efficient and specific protease of 27 kDa that has become a valuable biotechnological tool. Nowadays TEVp is a unique endopeptidase largely exploited in biotechnology from industrial applications to in vitro and in vivo cellular studies. A number of TEVp mutants with different rate of cleavage, stability and specificity have been reported. Similarly, a panel of different target cleavage sites, derived from the canonical ENLYFQ-G/S site, has been established. In this review we describe these aspects of TEVp and some of its multiple applications. A particular focus is on the use and molecular biology of TEVp in living cells and organisms. (C) 2016 Elsevier B.V. All rights reserved.
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页码：239 / 249
页数：11
相关论文
共 106 条
[11]   PROCESSING OF THE TOBACCO ETCH VIRUS 49K PROTEASE REQUIRES AUTOPROTEOLYSIS [J].
CARRINGTON, JC ;
DOUGHERTY, WG .
VIROLOGY, 1987, 160 (02) :355-362
[12]   SMALL NUCLEAR INCLUSION PROTEIN ENCODED BY A PLANT POTYVIRUS GENOME IS A PROTEASE [J].
CARRINGTON, JC ;
DOUGHERTY, WG .
JOURNAL OF VIROLOGY, 1987, 61 (08) :2540-2548
[13]   A VIRAL CLEAVAGE SITE CASSETTE - IDENTIFICATION OF AMINO-ACID SEQUENCES REQUIRED FOR TOBACCO ETCH VIRUS POLYPROTEIN PROCESSING [J].
CARRINGTON, JC ;
DOUGHERTY, WG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (10) :3391-3395
[14]   Simultaneous accumulation of multiple viral coat proteins from a TEV-NIa based expression vector [J].
Ceriani, MF ;
Marcos, JF ;
Hopp, HE ;
Beachy, RN .
PLANT MOLECULAR BIOLOGY, 1998, 36 (02) :239-248
[15]   Engineered tobacco etch virus (TEV) protease active in the secretory pathway of mammalian cells [J].
Cesaratto, Francesca ;
Lopez-Requena, Alejandro ;
Burronea, Oscar R. ;
Petris, Gianluca .
JOURNAL OF BIOTECHNOLOGY, 2015, 212 :159-166
[16]   Development of a prokaryotic-like polycistronic baculovirus expression vector by the linkage of two internal ribosome entry sites [J].
Chen, Wen-Shuo ;
Chang, Yen-Chung ;
Chen, Ying-Ju ;
Chen, Yu-Jie ;
Teng, Chao-Yi ;
Wang, Chung-Hsiung ;
Wu, Tzong-Yuan .
JOURNAL OF VIROLOGICAL METHODS, 2009, 159 (02) :152-159
[17]   TEV protease-facilitated stoichiometric delivery of multiple genes using a single expression vector [J].
Chen, Xi ;
Pham, Elizabeth ;
Truong, Kevin .
PROTEIN SCIENCE, 2010, 19 (12) :2379-2388
[18]  
Copeland MF, 2016, NAT CHEM BIOL, V12, P254, DOI [10.1038/NCHEMBIO.2021, 10.1038/nchembio.2021]
[19]   Application of TALEs, CRISPR/Cas and sRNAs as trans-acting regulators in prokaryotes [J].
Copeland, Matthew F. ;
Politz, Mark C. ;
Pfleger, Brian F. .
CURRENT OPINION IN BIOTECHNOLOGY, 2014, 29 :46-54
[20]   E unum pluribus:: multiple proteins from a self-processing polyprotein [J].
de Felipe, P ;
Luke, GA ;
Hughes, LE ;
Gani, D ;
Halpin, C ;
Ryan, MD .
TRENDS IN BIOTECHNOLOGY, 2006, 24 (02) :68-75
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