Endothelial Cell-Derived Endothelin-1 Promotes Cardiac Fibrosis in Diabetic Hearts Through Stimulation of Endothelial-to-Mesenchymal Transition

被引:335
作者
Widyantoro, Bambang
Emoto, Noriaki [1 ,3 ]
Nakayama, Kazuhiko
Anggrahini, Dyah W.
Adiarto, Suko
Iwasa, Naoko
Yagi, Keiko [3 ]
Miyagawa, Kazuya
Rikitake, Yoshiyuki [2 ]
Suzuki, Takashi [4 ]
Kisanuki, Yaz Y. [5 ]
Yanagisawa, Masashi [6 ]
Hirata, Ken-ichi
机构
[1] Kobe Univ, Grad Sch Med, Dept Internal Med, Div Cardiovasc Med,Chuo Ku, Kobe, Hyogo 6500017, Japan
[2] Kobe Univ, Grad Sch Med, Dept Biochem & Mol Biol, Div Mol & Cellular Biol, Kobe, Hyogo 6500017, Japan
[3] Kobe Pharmaceut Univ, Dept Clin Pharm, Kobe, Hyogo 658, Japan
[4] Tohoku Univ, Grad Sch Med, Dept Pathol, Sendai, Miyagi 980, Japan
[5] Ohio State Univ, Coll Med, Dept Neurol, Columbus, OH 43210 USA
[6] Univ Texas SW Med Ctr Dallas, Howard Hughes Med Inst, Dallas, TX 75390 USA
关键词
cardiomyopathy; diabetes mellitus; endothelin; fibrosis; heart failure; GROWTH-FACTOR; RECEPTOR ANTAGONISTS; EXPRESSION; CARDIOMYOPATHY; MYOCARDIUM; MELLITUS; TISSUE; FIBROBLASTS; ACTIVATION; INHIBITION;
D O I
10.1161/CIRCULATIONAHA.110.938217
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background-Persistently high plasma endothelin-1 (ET-1) levels in diabetic patients have been associated with the development of cardiac fibrosis, which results from the deposition of extracellular matrix and fibroblast recruitment from an as-yet unknown source. The underlying mechanism, however, remains elusive. Here, we hypothesize that ET-1 might contribute to the accumulation of cardiac fibroblasts through an endothelial-to-mesenchymal transition in diabetic hearts. Methods and Results-We induced diabetes mellitus in vascular endothelial cell-specific ET-1 knockout [ET-1(f/f); Tie2-Cre (+)] mice and their wild-type littermates using the toxin streptozotocin. Gene expression and histological and functional parameters were examined at 8, 24, and 36 weeks after the induction of diabetes mellitus. Diabetes mellitus increased cardiac ET-1 expression in wild-type mice, leading to mitochondrial disruption and myofibril disarray through the generation of superoxide. Diabetic mice also showed impairment of cardiac microvascularization and a decrease in cardiac vascular endothelial growth factor expression. ET-1 further promotes cardiac fibrosis and heart failure through the accumulation of fibroblasts via endothelial-to-mesenchymal transition. All of these features were abolished in ET-1(f/f); Tie2-Cre (+) hearts. Targeted ET-1 gene silencing by small interfering RNA in cultured human endothelial cells ameliorated high glucose-induced phenotypic transition and acquisition of a fibroblast marker through the inhibition of transforming growth factor-beta signaling activation and preservation of the endothelial cell-to-cell contact regulator VE-cadherin. Conclusions-These results provide new insights suggesting that diabetes mellitus-induced cardiac fibrosis is associated with the emergence of fibroblasts from endothelial cells and that this endothelial-to-mesenchymal transition process is stimulated by ET-1. Targeting endothelial cell-derived ET-1 might be beneficial in the prevention of diabetic cardiomyopathy. (Circulation. 2010; 121: 2407-2418.)
引用
收藏
页码:2407 / U88
页数:20
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