Derivation and culturing of neural stem cells from human brain tissue

被引：6

作者：

Suk, Yujin ^{[1
,2
]}

Kieliszek, Agata ^{[1
]}

Mobilio, Daniel ^{[1
]}

Venugopal, Chitra ^{[3
]}

Singh, Sheila K. ^{[1
,3
]}

机构：

[1] McMaster Univ, Dept Biochem & Biomed Sci, Hamilton, ON L8S 4L8, Canada

[2] McMaster Univ, Michael DeGroote Sch Med G, Hamilton, ON L8S 4L8, Canada

[3] McMaster Univ, Dept Surg, Hamilton, ON L8S 4L8, Canada

来源：

STAR PROTOCOLS | 2022年 / 3卷 / 03期

基金：

加拿大健康研究院;

关键词：

Cell culture; Cell isolation; Developmental biology; Health sciences; Neuroscience; Stem cells;

D O I：

10.1016/j.xpro.2022.101628

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human neural stem cells (hNSCs) are a valuable tool in brain cancer research since they are used as a normal control for multiple assays, mainly pertaining to toxicity. Here, we present a protocol to safely and successfully derive and culture hNSCs in vitro from human embryonic brain tissue. We describe the steps to dissociate embryonic brain tissue and culture hNSCs, followed by the procedure to expand hNSCs. These cells can be used for downstream applications including RNA-seq and omics studies. For complete details on the use and execution of this protocol, please refer to Venugopal et al. (2012b), Bakhshinyan et al. (2019), and Venugopal et al. (2012a).

引用

页数：9

共 50 条

[41] Trophoblast Stem Cell Derivation from Human Embryonic Stem Cells
Adachi, Katsuyuki
Yang, Ying
Ezashi, Toshihiko
Shultz, Laura C.
Schust, Danny J.
Roberts, R. M.
REPRODUCTIVE SCIENCES, 2014, 21 (03) : 104A - 105A
[42] Induction of Early Neural Precursors and Derivation of Tripotent Neural Stem Cells From Human Pluripotent Stem Cells Under Xeno-Free Conditions
Nguyen, Hal X.
Nekanti, Usha
Haus, Daniel L.
Funes, Gabrielle
Moreno, Denisse
Kamei, Noriko
Cummings, Brian J.
Anderson, Aileen J.
JOURNAL OF COMPARATIVE NEUROLOGY, 2014, 522 (12) : 2767 - 2783
[43] A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue
Shelley, Brandon C.
Gowing, Genevieve
Svendsen, Clive N.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2014, (88):
[44] A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue
Shelley, Brandon C.
Gowing, Genevieve
Svendsen, Clive N.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2014, (91):
[45] Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue
Lu, Jie
Delli-Bovi, Laurent C.
Hecht, Jonathan
Folkerth, Rebecca
Sheen, Volney L.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2011, (51):
[46] Human neural stem cells for traumatic brain injury
Gao, J
Parsley, MO
Taresenko, YI
Prough, DS
McAdoo, DJ
Wu, P
JOURNAL OF NEUROTRAUMA, 2005, 22 (10) : 1235 - 1235
[47] Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells
Pong, Sovannarath
Lizano, Paulo
Karmacharya, Rakesh
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2020, (165):
[48] Efficient derivation of human trophoblast stem cells from primed pluripotent stem cells
Wei, Yanxing
Wang, Tianyu
Ma, Lishi
Zhang, Yanqi
Zhao, Yuan
Lye, Kathryn
Xiao, Lu
Chen, Chunlin
Wang, Zhijian
Ma, Yanlin
Zhou, Xiaohua
Sun, Fei
Li, Weili
Dunk, Caroline
Li, Siliang
Nagy, Andras
Yu, Yanhong
Pan, Guangjin
Lye, Stephen J.
Shan, Yongli
SCIENCE ADVANCES, 2021, 7 (33)
[49] Analysis of Neural Stem Cells from Human Cortical Brain Structures In Vitro
M. A. Aleksandrova
R. A. Poltavtseva
M. V. Marei
G. T. Sukhikh
Bulletin of Experimental Biology and Medicine, 2016, 161 : 197 - 208
[50] Formation of Neuroepithelial Structures in Culture of Neural Stem Cells from Human Brain
O. V. Podgornyi
R. A. Poltavtseva
M. V. Marei
G. T. Sukhikh
M. A. Aleksandrova
Bulletin of Experimental Biology and Medicine, 2005, 140 : 113 - 117

← 1 2 3 4 5 →