Derivation and culturing of neural stem cells from human brain tissue

被引：6
|
作者：
Suk, Yujin [1 ,2 ]
Kieliszek, Agata [1 ]
Mobilio, Daniel [1 ]
Venugopal, Chitra [3 ]
Singh, Sheila K. [1 ,3 ]
机构：
[1] McMaster Univ, Dept Biochem & Biomed Sci, Hamilton, ON L8S 4L8, Canada
[2] McMaster Univ, Michael DeGroote Sch Med G, Hamilton, ON L8S 4L8, Canada
[3] McMaster Univ, Dept Surg, Hamilton, ON L8S 4L8, Canada
来源：
STAR PROTOCOLS | 2022年 / 3卷 / 03期
基金：
加拿大健康研究院;
关键词：
Cell culture; Cell isolation; Developmental biology; Health sciences; Neuroscience; Stem cells;
D O I：
10.1016/j.xpro.2022.101628
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Human neural stem cells (hNSCs) are a valuable tool in brain cancer research since they are used as a normal control for multiple assays, mainly pertaining to toxicity. Here, we present a protocol to safely and successfully derive and culture hNSCs in vitro from human embryonic brain tissue. We describe the steps to dissociate embryonic brain tissue and culture hNSCs, followed by the procedure to expand hNSCs. These cells can be used for downstream applications including RNA-seq and omics studies. For complete details on the use and execution of this protocol, please refer to Venugopal et al. (2012b), Bakhshinyan et al. (2019), and Venugopal et al. (2012a).
引用
收藏
页数：9
相关论文
共 50 条
  • [31] Derivation of trophoblast stem cells from naive human pluripotent stem cells
    Dong, Chen
    Beltcheva, Mariana
    Gontarz, Paul
    Zhang, Bo
    Popli, Pooja
    Fischer, Laura A.
    Khan, Shafqat A.
    Park, Kyoung-mi
    Yoon, Eun-Ja
    Xing, Xiaoyun
    Kommagani, Ramakrishna
    Wang, Ting
    Solnica-Krezel, Lilianna
    Theunissen, Thorold W.
    ELIFE, 2020, 9
  • [32] Derivation of human ES cell-derived neural stem cells
    Koch, P.
    Driehaus, J.
    Opitz, T.
    Ladewig, J.
    Steinbeck, J.
    Biegler, A.
    Steinfarz, B.
    Bruestle, O.
    ACTA NEUROPATHOLOGICA, 2006, 112 (03) : 356 - 357
  • [33] Improved Survival and Regeneration of Irradiated Mouse Neural Stem Cells after Co-Culturing with Non-Irradiated Mouse Neural Stem Cells or Mesenchymal Stem Cells from the Adipose Tissue
    Ratushnyak, M. G.
    Semochkina, Yu P.
    Zhirnik, A. S.
    Smirnova, O. D.
    BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 2021, 172 (02) : 228 - 235
  • [34] Efficient and Rapid Derivation of Primitive Neural Stem Cells and Generation of Brain Subtype Neurons From Human Pluripotent Stem Cells (vol 2, pg 862, 2013)
    Yan, Yiping
    Shin, Soojung
    Jha, Balendu Shekhar
    Liu, Qiuyue
    Sheng, Jianting
    Li, Fuhai
    Zhan, Ming
    Davis, Janine
    Bharti, Kapil
    Zeng, Xianmin
    Rao, Mahendra
    Malik, Nasir
    Vemuri, Mohan C.
    STEM CELLS TRANSLATIONAL MEDICINE, 2013, 2 (12) : 1022 - 1022
  • [35] Improved Survival and Regeneration of Irradiated Mouse Neural Stem Cells after Co-Culturing with Non-Irradiated Mouse Neural Stem Cells or Mesenchymal Stem Cells from the Adipose Tissue
    M. G. Ratushnyak
    Yu. P. Semochkina
    A. S. Zhirnik
    O. D. Smirnova
    Bulletin of Experimental Biology and Medicine, 2021, 172 : 228 - 235
  • [36] Derivation of sensory neurons and neural crest stem cells from human neural progenitor hNP1
    Guo, Xiufang
    Spradling, Severo
    Stancescu, Maria
    Lambert, Stephen
    Hickman, James J.
    BIOMATERIALS, 2013, 34 (18) : 4418 - 4427
  • [37] Derivation of adipocytes from human embryonic stem cells
    Xiong, C
    Xie, CQ
    Zhang, L
    Zhang, JF
    Xu, KF
    Fu, MG
    Thompson, WE
    Yang, LJ
    Chen, YQE
    STEM CELLS AND DEVELOPMENT, 2005, 14 (06) : 671 - 675
  • [38] Derivation of striatal neurons from human stem cells
    Viegas, Pedro
    Nicoleau, Camille
    Perrier, Anseime L.
    FUNCTIONAL NEURAL TRANSPLANTATION III PRIMARY AND STEM CELL THERAPIES FOR BRAIN REPAIR, PT I, 2012, 200 : 373 - 404
  • [39] Derivation of Neural Stem Cells from Mesenchymal Stem Cells: Evidence for a Bipotential Stem Cell Population
    Fu, Lijuan
    Zhu, Lunjian
    Huang, Yu
    Lee, Tsung D.
    Forman, Stephen J.
    Shih, Chu-Chih
    STEM CELLS AND DEVELOPMENT, 2008, 17 (06) : 1109 - 1121
  • [40] Brain tumour stem cells: the undercurrents of human brain cancer and their relationship to neural stem cells
    Dirks, Peter B.
    PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2008, 363 (1489) : 139 - 152
12345