Derivation and culturing of neural stem cells from human brain tissue

被引：6

作者：

Suk, Yujin ^{[1
,2
]}

Kieliszek, Agata ^{[1
]}

Mobilio, Daniel ^{[1
]}

Venugopal, Chitra ^{[3
]}

Singh, Sheila K. ^{[1
,3
]}

机构：

[1] McMaster Univ, Dept Biochem & Biomed Sci, Hamilton, ON L8S 4L8, Canada

[2] McMaster Univ, Michael DeGroote Sch Med G, Hamilton, ON L8S 4L8, Canada

[3] McMaster Univ, Dept Surg, Hamilton, ON L8S 4L8, Canada

来源：

STAR PROTOCOLS | 2022年 / 3卷 / 03期

基金：

加拿大健康研究院;

关键词：

Cell culture; Cell isolation; Developmental biology; Health sciences; Neuroscience; Stem cells;

D O I：

10.1016/j.xpro.2022.101628

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human neural stem cells (hNSCs) are a valuable tool in brain cancer research since they are used as a normal control for multiple assays, mainly pertaining to toxicity. Here, we present a protocol to safely and successfully derive and culture hNSCs in vitro from human embryonic brain tissue. We describe the steps to dissociate embryonic brain tissue and culture hNSCs, followed by the procedure to expand hNSCs. These cells can be used for downstream applications including RNA-seq and omics studies. For complete details on the use and execution of this protocol, please refer to Venugopal et al. (2012b), Bakhshinyan et al. (2019), and Venugopal et al. (2012a).

引用

页数：9

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