Electrophysiological assessment of primary cortical neurons genetically engineered using iron oxide nanoparticles

被引:4
作者
Evans, Michael G. [1 ]
Al-Shakli, Arwa [2 ]
Jenkins, Stuart I. [2 ]
Chari, Divya M. [2 ]
机构
[1] Keele Univ, Sch Life Sci, Keele ST5 5BG, Staffs, England
[2] Keele Univ, Inst Sci & Technol Med, Keele ST5 5BG, Staffs, England
基金
英国工程与自然科学研究理事会;
关键词
transfection; magnetic nanoparticle; green fluorescent protein (GFP); whole-cell patch clamp; fluorescence microscopy; SERUM-FREE CULTURE; TRANSFECTION; GENE; DNA; REGENERATION; ASTROCYTES; DELIVERY; GROWTH; CELLS; CNS;
D O I
10.1007/s12274-017-1496-4
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The development of safe technologies to genetically modify neurons is of great interest in regenerative neurology, for both translational and basic science applications. Such approaches have conventionally been heavily reliant on viral transduction methods, which have safety and production limitations. Magnetofection (magnet-assisted gene transfer using iron oxide nanoparticles as vectors) has emerged as a highly promising non-viral alternative for safe and reproducible genetic modification of neurons. Despite the high potential of this technology, there is an important gap in our knowledge of the safety of this approach, namely, whether it alters neuronal function in adverse ways, such as by altering neuronal excitability and signaling. We have investigated the effects of magnetofection in primary cortical neurons by examining neuronal excitability using the whole cell patch clamp technique. We found no evidence that magnetofection alters the voltage-dependent sodium and potassium ionic currents that underpin excitability. Our study provides important new data supporting magnetofection as a safe technology for bioengineering of neuronal cell populations.
引用
收藏
页码:2881 / 2890
页数:10
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