Fast identification of dermatophytes by MALDI-TOF/MS using direct transfer of fungal cells on ground steel target plates

被引:24
作者
da Cunha, Keith C. [1 ]
Riat, Arnaud [2 ]
Normand, Anne-Cecile [3 ,7 ]
Bosshard, Philipp P. [4 ,5 ]
de Almeida, Margarete T. G. [6 ]
Piarroux, Renaud [3 ,7 ]
Schrenzel, Jacques [2 ]
Fontao, Lionel [1 ]
机构
[1] Univ Hosp Geneva, Dermatol Lab, Div Lab Med, Geneva, Switzerland
[2] Univ Hosp Geneva, Bacteriol Lab, Div Lab Med, Geneva, Switzerland
[3] Univ Aix Marseille, Lab Parasitol Mycol, CHU Timone, Marseille, France
[4] Univ Hosp Zurich, Dept Dermatol, Zurich, Switzerland
[5] Univ Zurich, Fac Med, Zurich, Switzerland
[6] Fac Med Sao Jose do Rio Preto, Dept Infect & Parasit Dis, Sao Paulo, Brazil
[7] Grp Hosp Pitie Salpetriere, AP HP, Serv Parasitol Mycol, Paris, France
关键词
Dermatophytes; MALDI-TOF; Mass spectroscopy; FLIGHT MASS-SPECTROMETRY; DESORPTION-IONIZATION-TIME; ACCURATE IDENTIFICATION; SPECIES IDENTIFICATION; FILAMENTOUS FUNGI; SPACER REGIONS; MS SYSTEM; DIFFERENTIATION; INTERDIGITALE; RELEVANT;
D O I
10.1111/myc.12793
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Dermatophytes cause human infections limited to keratinised tissues. We showed that the direct transfer method allows reliable identification of non-dermatophytes mould and yeast by MALDI-TOF/MS. We aimed at assessing whether the direct transfer method can be used for dermatophytes and whether an own mass spectra library would be superior to the Bruker library. We used the Bruker Biotyper to build a dermatophyte mass spectra library and assessed its performance by 1/testing a panel of mass spectrum produced with strains genotypically identified and, 2/comparing MALDI-TOF/MS identification to morphology-based methods. Identification of dermatophytes using the Bruker library is poor. Our library provided 97% concordance between ITS sequencing and MALDI-TOF/MS analysis with a panel of 1104 spectra corresponding to 276 strains. Direct transfer method using unpolished target plates allowed proper identification of 85% of dermatophytes clinical isolates most of which were common dermatophytes. A homemade dermatophyte MSP library is a prerequisite for accurate identification of species absent in the Bruker library but it also improves identification of species already listed in the database. The direct deposit method can be used to identify the most commonly found dermatophytes such as T.rubrum and T.interdigitale/mentagrophytes by MALDI-TOF/MS.
引用
收藏
页码:691 / 697
页数:7
相关论文
共 23 条
[1]   Efficacy of terbinafine treatment of tinea capitis in children varies according to the dermatophyte species [J].
BaudrazRosselet, F ;
Monod, M ;
Jaccoud, S ;
Frenk, E .
BRITISH JOURNAL OF DERMATOLOGY, 1996, 135 (06) :1011-1012
[2]   Identification of Dermatophyte Species after Implementation of the In-House MALDI-TOF MS Database [J].
Calderaro, Adriana ;
Motta, Federica ;
Montecchini, Sara ;
Gorrini, Chiara ;
Piccolo, Giovanna ;
Piergianni, Maddalena ;
Buttrini, Mirko ;
Medici, Maria Cristina ;
Arcangeletti, Maria Cristina ;
Chezzi, Carlo ;
De Conto, Flora .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2014, 15 (09) :16012-16024
[3]   Performance of MALDI-TOF MS platforms for fungal identification [J].
Cassagne, Carole ;
Normand, Anne-Cecile ;
L'Ollivier, Coralie ;
Ranque, Stephane ;
Piarroux, Renaud .
MYCOSES, 2016, 59 (11) :678-690
[4]   Toward a Novel Multilocus Phylogenetic Taxonomy for the Dermatophytes [J].
de Hoog, G. Sybren ;
Dukik, Karolina ;
Monod, Michel ;
Packeu, Ann ;
Stubbe, Dirk ;
Hendrickx, Marijke ;
Kupsch, Christiane ;
Stielow, J. Benjamin ;
Freeke, Joanna ;
Goeker, Markus ;
Rezaei-Matehkolaei, Ali ;
Mirhendi, Hossein ;
Graeser, Yvonne .
MYCOPATHOLOGIA, 2017, 182 (1-2) :5-31
[5]   Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry Using the Vitek MS System for Rapid and Accurate Identification of Dermatophytes on Solid Cultures [J].
De Respinis, Sophie ;
Monnin, Valerie ;
Girard, Victoria ;
Welker, Martin ;
Arsac, Maud ;
Celliere, Beatrice ;
Durand, Geraldine ;
Bosshard, Philipp P. ;
Farina, Claudio ;
Passera, Marco ;
Van Belkum, Alex ;
Petrini, Orlando ;
Tonolla, Mauro .
JOURNAL OF CLINICAL MICROBIOLOGY, 2014, 52 (12) :4286-4292
[6]   Identification of dermatophytes by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry [J].
De Respinis, Sophie ;
Tonolla, Mauro ;
Pranghofer, Sigrid ;
Petrini, Liliane ;
Petrini, Orlando ;
Bosshard, Philipp P. .
MEDICAL MYCOLOGY, 2013, 51 (05) :514-521
[7]   Identification of dermatophyte species causing onychomycosis and tinea pedis by MALDI-TOF mass spectrometry [J].
Erhard, Marcel ;
Hipler, Uta-Christina ;
Burmester, Anke ;
Brakhage, Axel A. ;
Woestemeyer, Johannes .
EXPERIMENTAL DERMATOLOGY, 2008, 17 (04) :356-361
[8]   Matrix-assisted laser desorption ionization time-of-flight mass spectrometry: revolutionizing clinical laboratory diagnosis of mould infections [J].
Gautier, M. ;
Ranque, S. ;
Normand, A. -C. ;
Becker, P. ;
Packeu, A. ;
Cassagne, C. ;
L'Ollivier, C. ;
Hendrickx, M. ;
Piarroux, R. .
CLINICAL MICROBIOLOGY AND INFECTION, 2014, 20 (12) :1366-1371
[9]   Signature polymorphisms in the internal transcribed spacer region relevant for the differentiation of zoophilic and anthropophilic strains of Trichophyton interdigitale and other species of T. mentagrophytes sensu lato [J].
Heidemann, S. ;
Monod, M. ;
Graeser, Y. .
BRITISH JOURNAL OF DERMATOLOGY, 2010, 162 (02) :282-295
[10]   Species identification and strain differentiation of dermatophyte fungi by analysis of ribosomal-DNA intergenic spacer regions [J].
Jackson, CJ ;
Barton, RC ;
Evans, EGV .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (04) :931-936