LLM-Domain B-GATA Transcription Factors Promote Stomatal Development Downstream of Light Signaling Pathways in Arabidopsis thaliana Hypocotyls

被引:70
作者
Klermund, Carina [1 ]
Ranftl, Quirin L. [1 ]
Diener, Julia [1 ]
Bastakis, Emmanouil [1 ]
Richter, Rene [1 ,2 ]
Schwechheimer, Claus [1 ]
机构
[1] Tech Univ Munich, Plant Syst Biol, D-85354 Freising Weihenstephan, Germany
[2] Max Planck Inst Plant Breeding Res, Carl von Linne Weg 10, D-50829 Cologne, Germany
关键词
PHYTOCHROME-B; FLOWER DEVELOPMENT; SECRETORY PEPTIDE; CELL-DIVISION; HANABA TARANU; FACTORS GNC; DIFFERENTIATION; FAMILY; SPECIFICATION; GENE;
D O I
10.1105/tpc.15.00783
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Stomata are pores that regulate the gas and water exchange between the environment and aboveground plant tissues, including hypocotyls, leaves, and stems. Here, we show that mutants of Arabidopsis thaliana LLM-domain B-GATA genes are defective in stomata formation in hypocotyls. Conversely, stomata formation is strongly promoted by overexpression of various LLM-domain B-class GATA genes, most strikingly in hypocotyls but also in cotyledons. Genetic analyses indicate that these B-GATAs act upstream of the stomata formation regulators SPEECHLESS (SPCH), MUTE, and SCREAM/SCREAM2 and downstream or independent of the patterning regulators TOO MANY MOUTHS and STOMATAL DENSITY AND DISTRIBUTION1. The effects of the GATAs on stomata formation are light dependent but can be induced in dark-grown seedlings by red, far-red, or blue light treatments. PHYTOCHROME INTERACTING FACTOR (PIF) mutants form stomata in the dark, and in this genetic background, GATA expression is sufficient to induce stomata formation in the dark. Since the expression of the LLM-domain B-GATAs GNC (GATA, NITRATE-INDUCIBLE, CARBON METABOLISM-INVOLVED) and GNC-LIKE/CYTOKININ-RESPONSIVE GATA FACTOR1 as well as that of SPCH is red light induced but the induction of SPCH is compromised in a GATA gene mutant background, we hypothesize that PIF- and light-regulated stomata formation in hypocotyls is critically dependent on LLM-domain B-GATA genes.
引用
收藏
页码:646 / 660
页数:15
相关论文
共 56 条
[51]   Stomagen positively regulates stomatal density in Arabidopsis [J].
Sugano, Shigeo S. ;
Shimada, Tomoo ;
Imai, Yu ;
Okawa, Katsuya ;
Tamai, Atsushi ;
Mori, Masashi ;
Hara-Nishimura, Ikuko .
NATURE, 2010, 463 (7278) :241-U130
[52]   Organ-specific effects of brassinosteroids on stomatal production coordinate with the action of TOO MANY MOUTHS [J].
Wang, Ming ;
Yang, Kezhen ;
Le, Jie .
JOURNAL OF INTEGRATIVE PLANT BIOLOGY, 2015, 57 (03) :247-255
[53]  
Wengier D L, 2012, Cold Spring Harb Symp Quant Biol, V77, P53, DOI 10.1101/sqb.2013.77.015883
[54]   The too many mouths and four lips mutations affect stomatal production in arabidopsis [J].
Yang, M ;
Sack, FD .
PLANT CELL, 1995, 7 (12) :2227-2239
[55]   Transcription Repressor HANABA TARANU Controls Flower Development by Integrating the Actions of Multiple Hormones, Floral Organ Specification Genes, and GATA3 Family Genes in Arabidopsis [J].
Zhang, Xiaolan ;
Zhou, Yun ;
Ding, Lian ;
Wu, Zhigang ;
Liu, Renyi ;
Meyerowitz, Elliot M. .
PLANT CELL, 2013, 25 (01) :83-101
[56]   HANABA TARANU is a GATA transcription factor that regulates shoot apical meristem and flower development in Arabidopsis [J].
Zhao, YX ;
Medrano, L ;
Ohashi, K ;
Fletcher, JC ;
Yu, H ;
Sakai, H ;
Meyerowitz, EM .
PLANT CELL, 2004, 16 (10) :2586-2600