Abnormal Levels of Transcript Abundance of Developmentally Important Genes in Various Stages of Preimplantation Bovine Somatic Cell Nuclear Transfer Embryos

被引:23
|
作者
Aston, Kenneth I. [1 ,2 ]
Li, Guan-Peng [1 ,2 ]
Hicks, Brady A. [4 ]
Sessions, Benjamin R. [1 ,2 ]
Davis, Aaron P. [1 ,2 ]
Rickords, Lee F. [1 ,2 ]
Stevens, John R. [3 ]
White, Kenneth L. [1 ,2 ]
机构
[1] Utah State Univ, Dept Anim Dairy & Vet Sci, Logan, UT 84322 USA
[2] Utah State Univ, Ctr Integrated Biosyst, Logan, UT 84322 USA
[3] Utah State Univ, Dept Math & Stat, Logan, UT 84322 USA
[4] JR Simplot Co, Cattle Reprod Facil, Boise, ID USA
关键词
MAMMALIAN DEVELOPMENT; METHYLATION PATTERNS; EXPRESSION PATTERNS; FACTOR AP-2-GAMMA; MESSENGER-RNA; DONOR CELLS; CATTLE; PROFILES; CALVES; ADULT;
D O I
10.1089/cell.2009.0042
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Based on microarray data comparing gene expression of fibroblast donor cells and bovine somatic cell nuclear transfer (SCNT) and in vivo produced (AI) blastocysts, a group of genes including several transcription factors was selected for evaluation of transcript abundance. Using SYBR green-based real-time polymerase chain reaction (Q-PCR) the levels of POU domain class 5 transcription factor (Oct4), snail homolog 2 (Snai2), annexin A1 (Anxa1), thrombospondin (Thbs), tumor-associated calcium signal transducer 1 (Tacstd1), and transcription factor AP2 gamma (Tfap2c) were evaluated in bovine fibroblasts, oocytes, embryos 30 min postfusion (SCNT), 12 h postfertilization/activation, as well as two-cell, four-cell, eight-cell, morula, and blastocyst-stage in vitro fertilized (IVF) and SCNT embryos. For every gene except Oct4, levels of transcript were indistinguishable between IVF and SCNT embryos at the blastocyst stage; however, in many cases levels of these genes during stages prior to blastocyst differed significantly. Altered levels of gene transcripts early in development likely have developmental consequences downstream. These results indicate that experiments evaluating gene expression differences between control and SCNT blastocysts may underestimate the degree of difference between clones and controls, and further offer insights into the dynamics of transcript regulation following SCNT.
引用
收藏
页码:23 / 32
页数:10
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