Human cerebrospinal fluid increases the excitability of pyramidal neurons in the in vitro brain slice

被引:28
作者
Bjorefeldt, Andreas [1 ]
Andreasson, Ulf [2 ]
Daborg, Jonny [1 ]
Riebe, Ilse [1 ]
Wasling, Pontus [3 ]
Zetterberg, Henrik [2 ,4 ]
Hanse, Eric [1 ]
机构
[1] Univ Gothenburg, Inst Neurosci & Physiol, Dept Physiol, S-40530 Gothenburg, Sweden
[2] Univ Gothenburg, Inst Neurosci & Physiol, Dept Psychiat & Neurochem, S-43180 Molndal, Sweden
[3] Univ Gothenburg, Inst Neurosci & Physiol, Dept Clin Neurosci, S-41345 Gothenburg, Sweden
[4] UCL Inst Neurol, London WC1N 3BG, England
来源
JOURNAL OF PHYSIOLOGY-LONDON | 2015年 / 593卷 / 01期
基金
瑞典研究理事会;
关键词
NEOCORTICAL NEURONS; CONTACTING NEURONS; RAT; CORTEX; METABOLITES; MONOAMINE; GLUTAMATE; CHANNELS; HORMONE; BARRIER;
D O I
10.1113/jphysiol.2014.284711
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The composition of brain extracellular fluid is shaped by a continuous exchange of substances between the cerebrospinal fluid (CSF) and interstitial fluid. The CSF is known to contain a wide range of endogenous neuromodulatory substances, but their collective influence on neuronal activity has been poorly investigated. We show here that replacing artificial CSF (aCSF), routinely used for perfusion of brain slices in vitro, with human CSF (hCSF) powerfully boosts spontaneous firing of CA1, CA3 and layer 5 pyramidal neurons in the rat brain slice. CA1 pyramidal neurons in hCSF display lowered firing thresholds, more depolarized resting membrane potentials and reduced input resistance, mimicking properties of pyramidal neurons recorded in vivo. The increased excitability of CA1 pyramidal neurons was completely occluded by intracellular application of GTPS, suggesting that endogenous neuromodulators in hCSF act on G-protein coupled receptors to enhance excitability. We found no increase in spontaneous inhibitory synaptic transmission by hCSF, indicating a differential effect on glutamatergic and GABAergic neurons. Our findings highlight a previously unknown function of the CSF in promoting spontaneous excitatory activity, and may help to explain differences observed in the activity of pyramidal neurons recorded in vivo and in vitro.
引用
收藏
页码:231 / 243
页数:13
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