The Cdc48-Ufd1-Np14 complex is central in ubiquitin-proteasome triggered catabolite degradation of fructose-1,6-bisphosphatase

被引：12

作者：

Barbin, Lise ^{[1
]}

Eisele, Frederik ^{[1
]}

Santt, Olivier ^{[1
]}

Wolf, Dieter H. ^{[1
]}

机构：

[1] Univ Stuttgart, Inst Biochem, D-70569 Stuttgart, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 394卷 / 02期

关键词：

Protein degradation; Gluconeogenesis; Fructose-1,6-bisphosphatase; Cdc48; complex; Polyubiquitination; Proteasome; YEAST SACCHAROMYCES-CEREVISIAE; AAA ATPASE CDC48/P97; ENDOPLASMIC-RETICULUM; PROTEIN-DEGRADATION; PHOSPHOENOLPYRUVATE CARBOXYKINASE; TRANSCRIPTION FACTOR; UBX DOMAIN; INACTIVATION; ER; CHAPERONE;

D O I：

10.1016/j.bbrc.2010.03.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The switch from gluconeogenesis to glycolysis in yeast has been shown to require ubiquitin-proteasome dependent elimination of the key enzyme fructose-1,6-bisphosphatase (FBPase). Prior to proteasomal degradation, polyubiquitination of the enzyme occurs via the ubiquitin-conjugating enzymes Ubc1, Ubc4, Ubc5 and Ubc8 in conjunction with a novel multi-subunit ubiquitin ligase, the Gid complex. As an additional machinery required for the catabolite degradation process, we identified the trimeric Cdc48(Ufd1-Np14) complex and the ubiquitin receptors Dsk2 and Rad23. We show that this machinery acts between polyubiquitination of FBPase and its degradation by the proteasome. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：335 / 341

页数：7

共 59 条

[11] Cdc48p interacts with Ufd3p, a WD repeat protein required for ubiquitin-mediated proteolysis in Saccharomyces cerevisiae [J].

Ghislain, M ;

Dohmen, RJ ;

Levy, F ;

Varshavsky, A .

EMBO JOURNAL, 1996, 15 (18) :4884-4899

[12] A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast -: art. no. e23 [J].

Gueldener, U ;

Heinisch, J ;

Koehler, GJ ;

Voss, D ;

Hegemann, JH .

NUCLEIC ACIDS RESEARCH, 2002, 30 (06) :23

[13]

Guthrie C., 1991, METHODS ENZYMOLOGY

[14] Proteins of newly isolated mutants and the amino-terminal proline are essential for ubiquitin-proteasome-catalyzed catabolite degradation of fructose-1,6-bisphosphatase of Saccharomyces cerevisiae [J].

Hämmerle, M ;

Bauer, J ;

Rose, M ;

Szallies, A ;

Thumm, M ;

Düsterhus, S ;

Mecke, D ;

Entian, KD ;

Wolf, DH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (39) :25000-25005

[15] The conserved Np14 protein complex mediates proteasome-dependent membrane-bound transcription factor activation [J].

Hitchcock, AL ;

Krebber, H ;

Frietze, S ;

Lin, A ;

Latterich, M ;

Silver, PA .

MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (10) :3226-3241

[16] Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry [J].

Ho, Y ;

Gruhler, A ;

Heilbut, A ;

Bader, GD ;

Moore, L ;

Adams, SL ;

Millar, A ;

Taylor, P ;

Bennett, K ;

Boutilier, K ;

Yang, LY ;

Wolting, C ;

Donaldson, I ;

Schandorff, S ;

Shewnarane, J ;

Vo, M ;

Taggart, J ;

Goudreault, M ;

Muskat, B ;

Alfarano, C ;

Dewar, D ;

Lin, Z ;

Michalickova, K ;

Willems, AR ;

Sassi, H ;

Nielsen, PA ;

Rasmussen, KJ ;

Andersen, JR ;

Johansen, LE ;

Hansen, LH ;

Jespersen, H ;

Podtelejnikov, A ;

Nielsen, E ;

Crawford, J ;

Poulsen, V ;

Sorensen, BD ;

Matthiesen, J ;

Hendrickson, RC ;

Gleeson, F ;

Pawson, T ;

Moran, MF ;

Durocher, D ;

Mann, M ;

Hogue, CWV ;

Figeys, D ;

Tyers, M .

NATURE, 2002, 415 (6868) :180-183

[17]

HOLZER H, 1976, TRENDS BIOCHEM SCI, V1, P178, DOI 10.1016/0968-0004(76)90201-2

[18] Degradation of the gluconeogenic enzymes fructose-1,6-bisphosphatase and malate dehydrogenase is mediated by distinct proteolytic pathways and signaling events [J].

Hung, GC ;

Brown, CR ;

Wolfe, AB ;

Liu, JJ ;

Chiang, HL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (47) :49138-49150

[19] Protein dislocation from the ER requires polyubiquitination and the AAA-ATPase Cdc48 [J].

Jarosch, E ;

Taxis, C ;

Volkwein, C ;

Bordallo, J ;

Finley, D ;

Wolf, DH ;

Sommer, T .

NATURE CELL BIOLOGY, 2002, 4 (02) :134-139

[20] Cdc48 (p97): a 'molecular gearbox' in the ubiquitin pathway? [J].

Jentsch, Stefan ;

Rumpf, Sebastian .

TRENDS IN BIOCHEMICAL SCIENCES, 2007, 32 (01) :6-11

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