The Cdc48-Ufd1-Np14 complex is central in ubiquitin-proteasome triggered catabolite degradation of fructose-1,6-bisphosphatase

被引：12

作者：

Barbin, Lise ^{[1
]}

Eisele, Frederik ^{[1
]}

Santt, Olivier ^{[1
]}

Wolf, Dieter H. ^{[1
]}

机构：

[1] Univ Stuttgart, Inst Biochem, D-70569 Stuttgart, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 394卷 / 02期

关键词：

Protein degradation; Gluconeogenesis; Fructose-1,6-bisphosphatase; Cdc48; complex; Polyubiquitination; Proteasome; YEAST SACCHAROMYCES-CEREVISIAE; AAA ATPASE CDC48/P97; ENDOPLASMIC-RETICULUM; PROTEIN-DEGRADATION; PHOSPHOENOLPYRUVATE CARBOXYKINASE; TRANSCRIPTION FACTOR; UBX DOMAIN; INACTIVATION; ER; CHAPERONE;

D O I：

10.1016/j.bbrc.2010.03.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The switch from gluconeogenesis to glycolysis in yeast has been shown to require ubiquitin-proteasome dependent elimination of the key enzyme fructose-1,6-bisphosphatase (FBPase). Prior to proteasomal degradation, polyubiquitination of the enzyme occurs via the ubiquitin-conjugating enzymes Ubc1, Ubc4, Ubc5 and Ubc8 in conjunction with a novel multi-subunit ubiquitin ligase, the Gid complex. As an additional machinery required for the catabolite degradation process, we identified the trimeric Cdc48(Ufd1-Np14) complex and the ubiquitin receptors Dsk2 and Rad23. We show that this machinery acts between polyubiquitination of FBPase and its degradation by the proteasome. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：335 / 341

页数：7

共 59 条

[1] Ubx4 Modulates Cdc48 Activity and Influences Degradation of Misfolded Proteins of the Endoplasmic Reticulum [J].

Alberts, Sven M. ;

Sonntag, Caroline ;

Schaefer, Antje ;

Wolf, Dieter H. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (24) :16082-16089

[2]

Ausubel FM., 1994, Curr. Protoc. Mol. Biol

[3] HRD4/NPL4 is required for the proteasomal processing of ubiquitinated ER proteins [J].

Bays, NW ;

Wilhovsky, SK ;

Goradia, A ;

Hodgkiss-Harlow, K ;

Hampton, RY .

MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (12) :4114-4128

[4] Conformational changes in the AAA ATPase p97-p47 adaptor complex [J].

Beuron, Fabienne ;

Dreveny, Ingrid ;

Yuan, Xuemei ;

Pye, Valerie E. ;

McKeown, Ciaran ;

Briggs, Louise C. ;

Cliff, Matthew J. ;

Kaneko, Yayoi ;

Wallis, Russell ;

Isaacson, Rivka L. ;

Ladbury, John E. ;

Matthews, Steve J. ;

Kondo, Hisao ;

Zhang, Xiaodong ;

Freemont, Paul S. .

EMBO JOURNAL, 2006, 25 (09) :1967-1976

[5] Role of the ubiquitin-selective CDC48UFD1/NPL4 chaperone (segregase) in ERAD of OLE1 and other substrates [J].

Braun, S ;

Matuschewski, K ;

Rape, M ;

Thoms, S ;

Jentsch, S .

EMBO JOURNAL, 2002, 21 (04) :615-621

[6] REGULATED IMPORT AND DEGRADATION OF A CYTOSOLIC PROTEIN IN THE YEAST VACUOLE [J].

CHIANG, HL ;

SCHEKMAN, R .

NATURE, 1991, 350 (6316) :313-318

[7] Binding of Cdc48p to a ubiquitin-related UBX domain from novel yeast proteins involved in intracellular proteolysis and sporulation [J].

Decottignies, A ;

Evain, A ;

Ghislain, M .

YEAST, 2004, 21 (02) :127-139

[8] Mutants of the deubiquitinating enzyme Ubp14 decipher pathway diversity of ubiquitin-proteasome linked protein degradation [J].

Eisele, Frederik ;

Braun, Bernhard ;

Pfirrmann, Thorsten ;

Wolf, Dieter H. .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 350 (02) :329-333

[9] TURNOVER OF YEAST FRUCTOSE-BIS-PHOSPHATASE IN DIFFERENT METABOLIC CONDITIONS [J].

FUNAYAMA, S ;

GANCEDO, JM ;

GANCEDO, C .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1980, 109 (01) :61-66

[10] INACTIVATION OF FRUCTOSE-1,6-DIPHOSPHATASE BY GLUCOSE IN YEAST [J].

GANCEDO, C .

JOURNAL OF BACTERIOLOGY, 1971, 107 (02) :401-&

← 1 2 3 4 5 6 →