Interferon-τ modulates phorbol ester-induced production of prostaglandin and expression of cyclooxygenase-2 and phospholipase-A2 from bovine endometrial cells

被引:54
作者
Binelli, M
Guzeloglu, A
Badinga, L
Arnold, DR
Sirois, J
Hansen, TR
Thatcher, WW
机构
[1] Univ Florida, Dept Dairy & Poultry Sci, Gainesville, FL 32611 USA
[2] Univ Montreal, Fac Med Vet, Ctr Rech Reprod Anim, St Hyacinthe, PQ J2S 7C6, Canada
[3] Univ Wyoming, Dept Anim Sci, Laramie, WY 82071 USA
关键词
conceptus; hormone action; kinases; pregnancy; uterus;
D O I
10.1095/biolreprod63.2.417
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Antiluteolytic actions of bovine interferon-tau (bIFN-tau) require suppression of prostaglandin F-2 alpha (PGF(2 alpha)) production. Our objective was to test whether bIFN-tau could block PGF(2 alpha) production and synthesis of phospholipase A(2) (PLA(2)) and cyclooxygenase-2 (COX-2) enzymes induced by a protein kinase C (PKC) stimulator (phorbol 12,13 dibutyrate; PDBu). Bovine endometrial epithelial (BEND) cells were treated with PDBu in the presence or absence of bIFN-tau. Medium samples were analyzed for concentrations of PGF(2 alpha), whole-cell extracts were analyzed for abundance of PLA, and COX-2 by immunoblotting, and RNA extracts were examined for steady-state levels of COX-2 mRNA by Northern blotting. The PDBu stimulated production of PGF(2 alpha) between 3 and 12 h, levels of COX-2 mRNA by 3 h and protein expression of COX-2 and PLA, by 6 and 12 h, respectively, Added concomitantly with PDBu, bIFN-tau suppressed PGF(2 alpha) production, steady-state levels of COX-2 mRNA, and expression of COX-2 and PLA(2) proteins. Added after a 3-h stimulation with PDBu atone, bIFN-tau suppressed PGF(2 alpha) production after 1 h. Bovine IFN-tau inhibited intracellular mechanisms responsible for PCF2 alpha production in BEND cells, and this could be through both cytosolic and nuclear actions.
引用
收藏
页码:417 / 424
页数:8
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