The Influence of In Vitro Gastrointestinal Digestion on the Anticancer Activity of Manuka Honey

被引：32

作者：

Cianciosi, Danila ^{[1
]}

Yuliett Forbes-Hernandez, Tamara ^{[2
]}

Afrin, Sadia ^{[3
]}

Gasparrini, Massimiliano ^{[4
]}

Quiles, Jose L. ^{[5
,6
]}

Gil, Emilio ^{[7
]}

Bompadre, Stefano ^{[8
]}

Simal-Gandara, Jesus ^{[9
]}

Battino, Maurizio ^{[1
,2
,10
]}

Giampieri, Francesca ^{[1
,2
,6
]}

机构：

[1] Univ Politecn Marche, Dipartimento Sci Clin Specialist & Odontostomtolo, Via Ranieri 65, I-60130 Ancona, Italy

[2] Univ Vigo, CACTI, CITACA, Nutr & Food Sci Grp,Dept Analyt & Food Chem, Vigo Campus, Orense 32004, Spain

[3] Johns Hopkins Univ, Sch Med, Dept Gynecol & Obstet, Baltimore, MD 21205 USA

[4] Univ Politecn Marche, Dept Agr Food & Environm Sci, Via Brecce Bianche 10, I-60131 Ancona, Italy

[5] Univ Granada, Biomed Res Ctr, Inst Nutr & Food Technol Jose Mataix, Dept Physiol, Granada 18000, Spain

[6] Northwest Univ, Coll Food Sci & Technol, Xian 710069, Peoples R China

[7] Univ Vigo, Fac Biol, Dept Biochem Genet & Immunol, Nutr & Food Sci Grp, Vigo 36310, Spain

[8] Polytech Univ Marche, Dept Biomed Sci, I-60131 Ancona, Italy

[9] Univ Vigo, Fac Sci, CITACA, Nutr & Bromatol Grp,Dept Analyt & Food Chem, Ourense Campus, E-32004 Orense, Spain

[10] Jiangsu Univ, Int Res Ctr Food Nutr & Safety, Zhenjiang 212013, Jiangsu, Peoples R China

来源：

ANTIOXIDANTS | 2020年 / 9卷 / 01期

关键词：

bioaccessibility; bioavailability; colon cancer; honey; polyphenols; in vitro simulated digestion; ANTIOXIDANT ACTIVITY; CELL-CYCLE; PHENOLIC-COMPOUNDS; APOPTOSIS; BIOACCESSIBILITY; CAPACITY; POLYPHENOLS; EXTRACTS; GROWTH;

D O I：

10.3390/antiox9010064

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Manuka honey (MH) is a natural food with many beneficial properties to human health, thanks to its high variety of bioactive compounds; however, little is known about its bioaccessibility. The aim of this study was to evaluate and compare the polyphenol compounds, the antioxidant capacity and the anticancer activity of MH subjected to an in vitro gastrointestinal digestion in human HCT-116 colon cancer cells. Raw MH and digested MH (DMH) were assessed for total polyphenols and flavonoids by spectrophotometric and HPLC-ESI-MS/MS analysis, and total antioxidant capacity (TAC) using different methods. Cell viability, intracellular ROS production, apoptosis, cell cycle and colony formation capacity were tested after treatment with MH or DMH. Results showed that total polyphenols, total flavonoids and TAC were significantly (p < 0.05) reduced after in vitro digestion. In addition, MH and DMH at 8, 16 and 24 mg/mL had similar effects in inducing intracellular ROS production and in inhibiting the colon formation ability; MH induced a more marked apoptosis compared to DMH, while cell cycle was blocked in S phase by MH and in Sub G1 phase by DMH. Our results increase knowledge of the effect of gastrointestinal digestion on the biological effect of honey against colorectal cancer.

引用

页数：20

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