Synthesis and Characterization of Acylated Polycaprolactone (PCL) Nanospheres and Investigation of Their Influence on Aggregation of Amyloid Proteins

被引：15

作者：

Ansari, Mojtaba ^{[1
]}

Salahshour-Kordestani, Soheila ^{[1
]}

Habibi-Rezaei, Mehran ^{[2
,3
]}

Movahedi, Ali Akbar Moosavi ^{[4
,5
]}

机构：

[1] Amirkabir Univ Technol, Fac Biomed Engn, Biomat Grp, Tehran, Iran

[2] Univ Tehran, Sch Biol, Coll Sci, Tehran, Iran

[3] Univ Tehran, Nanobiomed Ctr Excellence Nanosci & Nanotechnol, Res Ctr, Tehran, Iran

[4] Univ Tehran, Inst Biochem & Biophys, Tehran, Iran

[5] Univ Tehran, Ctr Excellence Biothermodynam, Tehran, Iran

来源：

JOURNAL OF MACROMOLECULAR SCIENCE PART B-PHYSICS | 2015年 / 54卷 / 01期

关键词：

acylated polycaprolactone (PCL) nanospheres; Alzheimer's disease; amyloid aggregation; hydrophobic interaction; protein misfolding; thioflavin T(ThT); NANOPARTICLES; FIBRILLATION; PEPTIDE; SURFACE; SIZE;

D O I：

10.1080/00222348.2014.984578

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Acylated polycaprolactone (PCL) nanospheres were fabricated and employed to interact with amyloid-beta-(25-35) peptides (A beta(25-35)), "peptide 11 of the 40 peptide full amyloid-beta". The nanospheres were characterized by scanning electron microscopy (SEM), attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, and dynamiclightscattering (DLS), all of which confirmed that the acylated polycaprolactone (Ac-PCL) nanospheres were successfully fabricated. The effect of the nanospheres on the aggregation of A beta(25-35) peptides was investigated by thioflavin T fluorescence measurements. The result showed that, without nanospheres, the A beta(25-35) peptides aggregated gradually from monomers and oligomers to long fibrils with increasing incubation time. In comparison, the nanospheres were effective in interfering with fibrillogenesis and aggregation of amyloid-beta. We suggest this study may contribute to the development of new therapeutic strategies against amyloid-related disorders.

引用

页码：71 / 80

页数：10

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