Numerical simulation of near-field fluorescence correlation spectroscopy using a fiber probe

被引：1

作者：

Kasahara, Kazuo ^{[1
]}

Saiki, Toshiharu ^{[1
]}

机构：

[1] Keio Univ, Sch Integrated Design Engn, Kohoku Ku, Kanagawa 2238522, Japan

来源：

JOURNAL OF NANOPHOTONICS | 2010年 / 4卷

关键词：

Brownian motion; fluorescence spectroscopy; near-field scanning optical microscopy; optical correlation; DIFFUSION; MICROSCOPY;

D O I：

10.1117/1.3319569

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Numerical simulation of fluorescence correlation spectroscopy (FCS) based on near-field scanning optical microscopy (NSOM) was performed. A finite-difference time-domain method was employed to calculate the electric field distribution in the vicinity of the NSOM aperture and the Brownian motion of nanoparticles was reproduced by a Monte Carlo simulation. The physical validity of the simulation result was confirmed by comparing it with a theoretical calculation using a Gaussian observation volume, which is applied to FCS based on conventional optical microscopy.

引用

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