Characteristics of Trametes villosa laccase adsorbed on aluminum hydroxide

被引:38
作者
Ahn, Mi-Youn
Zimmerman, Andrew R.
Martinez, Carmen E.
Archibald, Douglas D.
Bollag, Jean-Marc
Dec, Jerzy
机构
[1] Penn State Univ, Soil Biochem Lab, Penn State Inst Environm, University Pk, PA 16802 USA
[2] Univ Florida, Dept Soil & Water Sci, Gainesville, FL 32611 USA
[3] Univ Florida, Dept Geol Sci, Gainesville, FL 32611 USA
[4] Penn State Univ, Dept Crop & Soil Sci, University Pk, PA 16802 USA
基金
美国国家科学基金会;
关键词
laccase; aluminum hydroxide; adsorption; enzyme activity; soil enzymes;
D O I
10.1016/j.enzmictec.2006.12.014
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The interaction of metal oxides with extracellular enzymes may influence humus formation and bioremediation processes. In this study, short-range ordered aluminum hydroxide exhibited a strong affinity for laccase from Trametes villosa (X-m = 6.18 mu g mg(-1)) as evidenced by an H-type adsorption isotherm. Based on oxygen uptake measurements, free laccase had only slightly greater kinetic parameters (Km = 0. 18 mm; V-max = 14.35 mmol O-2 min(-1)) than those of adsorbed laccase (K-m = 0.15 mm; V-max = 12.77 mmol O-2 min(-1)). Free and adsorbed laccase showed similar activities under various pH conditions (from 3.8 to 7.8) and also showed similar activity loss over time (0-30 days). Adsorbed laccase was less resistant to thermal (4-100 degrees C) and proteolytic degradation, but also less sensitive to inhibition by humic acid than free laccase. Attenuated total reflectance infrared (ATR-IR) spectroscopy did not show significant changes in the secondary structure of laccase due to adsorption on aluminum hydroxide. These results indicate that laccase in soil may be found predominantly complexed with short-range ordered aluminum hydroxide, and laccase activity and stability is little affected by adsorption to the mineral. Consequently, use of immobilized enzymes such as laccase on aluminum hydroxide can be considered a practical tool for soil remediation. (c) 2007 Elsevier Inc. All rights reserved.
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页码:141 / 148
页数:8
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