Monoclonal antibodies generated against recombinant ATM support kinase activity

被引：7
作者：
Alligood, KJ
Milla, M
Rhodes, N
Ellis, B
Kilpatrick, KE
Lee, A
Gilmer, TM
Lansing, TJ
机构：
[1] Glaxo Wellcome Inc, Res & Dev, Dept Canc Biol, Res Triangle Pk, NC 27709 USA
[2] Glaxo Wellcome Inc, Res & Dev, Dept Prot Sci, Res Triangle Pk, NC 27709 USA
来源：
HYBRIDOMA | 2000年 / 19卷 / 04期
关键词：
D O I：
10.1089/027245700429864
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We report on the rapid generation of two monoclonal antibodies, ATM A16.35 and ATM. D16.11, that bind to the kinase domain of mutated ataxia telangiectasia (ATM). These antibodies were generated against E. coli-expressed recombinant protein using the RIMMS strategy. We show that ATM A16.35 binds ATM by Western blot analysis, and ATM D16.11 forms immune complexes with native ATM in immunoprecipitations without neutralizing kinase activity.
引用
收藏
页码：317 / 321
页数：5
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