Site-specific incorporation of phosphotyrosine using an expanded genetic code

被引：98

作者：

Hoppmann, Christian ^{[1
]}

Wong, Allison ^{[2
]}

Yang, Bing ^{[1
]}

Li, Shuwei ^{[3
]}

Hunter, Tony ^{[4
]}

Shokat, Kevan M. ^{[2
]}

Wang, Lei ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA USA

[2] Univ Calif San Francisco, Cellular & Mol Pharmacol, San Francisco, CA 94143 USA

[3] Univ Maryland Coll Pk, Inst Biosci & Biotechnol Res, Rockville, MD USA

[4] Salk Inst Biol Studies, Mol & Cell Biol Lab, 10010 N Torrey Pines Rd, La Jolla, CA 92037 USA

来源：

NATURE CHEMICAL BIOLOGY | 2017年 / 13卷 / 08期

关键词：

TRANSFER-RNA SYNTHETASE; UNNATURAL AMINO-ACIDS; ESCHERICHIA-COLI; PROTEIN-PHOSPHORYLATION; REVEALS; PHOSPHOSERINE; SYSTEM; KINASE; ANALOG;

D O I：

10.1038/nchembio.2406

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Access to phosphoproteins with stoichiometric and site-specific phosphorylation status is key to understanding the role of protein phosphorylation. Here we report an efficient method to generate pure, active phosphotyrosine-containing proteins by genetically encoding a stable phosphotyrosine analog that is convertible to native phosphotyrosine. We demonstrate its general compatibility with proteins of various sizes, phosphotyrosine sites and functions, and reveal a possible role of tyrosine phosphorylation in negative regulation of ubiquitination.

引用

页码：842 / +

页数：5

共 30 条

[1] Oncogenic kinase signalling
Blume-Jensen, P
Hunter, T
[J]. NATURE, 2001, 411 (6835) : 355 - 365
[2] SYNTHESIS AND APPLICATION OF FMOC-O-[BIS(DIMETHYLAMINO)PHOSPHONO]TYROSINE, A VERSATILE PROTECTED PHOSPHOTYROSINE EQUIVALENT
CHAO, HG
LEITING, B
REISS, PD
BURKHARDT, AL
KLIMAS, CE
BOLEN, JB
MATSUEDA, GR
[J]. JOURNAL OF ORGANIC CHEMISTRY, 1995, 60 (24) : 7710 - 7711
[3] Synthetic approaches to protein phosphorylation
Chen, Zan
Cole, Philip A.
[J]. CURRENT OPINION IN CHEMICAL BIOLOGY, 2015, 28 : 115 - 122
[4] Pivotal role for the ubiquitin Y59-E51 loop in lysine 48 polyubiquitination
Chong, Robert A.
Wu, Kenneth
Spratt, Donald E.
Yang, Yingying
Lee, Chan
Nayak, Jaladhi
Xu, Ming
Elkholi, Rana
Tappin, Inger
Li, Jessica
Hurwitz, Jerard
Brown, Brian D.
Chipuk, Jerry Edward
Chen, Zhijian J.
Sanchez, Roberto
Shaw, Gary S.
Huang, Lan
Pan, Zhen-Qiang
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2014, 111 (23) : 8434 - 8439
[5] Expanding the genetic code of Escherichia coli with phosphotyrosine
Fan, Chenguang
Ip, Kevan
Soll, Dieter
[J]. FEBS LETTERS, 2016, 590 (17): : 3040 - 3047
[6] Generation of phospho-ubiquitin variants by orthogonal translation reveals codon skipping
George, Susanna
Aguirre, Jacob D.
Spratt, Donald E.
Bi, Yumin
Jeffery, Madeline
Shaw, Gary S.
O'Donoghue, Patrick
[J]. FEBS LETTERS, 2016, 590 (10): : 1530 - 1542
[7] Genetically Encoding Photoswitchable Click Amino Acids in Escherichia coli and Mammalian Cells
Hoppmann, Christian
Lacey, Vanessa K.
Louie, Gordon V.
Wei, Jing
Noel, Joseph P.
Wang, Lei
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2014, 53 (15) : 3932 - 3936
[8] PhosphoSitePlus: a comprehensive resource for investigating the structure and function of experimentally determined post-translational modifications in man and mouse
Hornbeck, Peter V.
Kornhauser, Jon M.
Tkachev, Sasha
Zhang, Bin
Skrzypek, Elzbieta
Murray, Beth
Latham, Vaughan
Sullivan, Michael
[J]. NUCLEIC ACIDS RESEARCH, 2012, 40 (D1) : D261 - D270
[9] Protein Phosphorylation: A Major Switch Mechanism for Metabolic Regulation
Humphrey, Sean J.
James, David E.
Mann, Matthias
[J]. TRENDS IN ENDOCRINOLOGY AND METABOLISM, 2015, 26 (12) : 676 - 687
[10] Tyrosine-phosphorylation: thirty years and counting
Hunter, Tony
[J]. CURRENT OPINION IN CELL BIOLOGY, 2009, 21 (02) : 140 - 146

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