Quantitative and qualitative study of enzyme-linked immunosorbent assay to detect IgG against Japanese encephalitis virus in swine sera

被引:18
作者
Jia, XL
Chen, HC [1 ]
Wang, X
Qi, CM
机构
[1] Huazhong Agr Univ, Coll Anim Sci & Vet Med, Wuhan 430071, Peoples R China
[2] China Agr Univ, Coll Vet Med, Beijing 100094, Peoples R China
关键词
enzyme-linked immunosorbent assay; haemagglutination inhibition; IgG; Japanese encephalitis virus; swine;
D O I
10.1023/B:VERC.0000047485.85866.6e
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
This study was designed to investigate the application of indirect enzyme-linked immunoassay (ELISA) in detecting IgG against Japanese encephalitis virus in swine sera and the qualitative nature of this test. The attenuated strain SA14-14-2 of Japanese encephalitis virus (JEV) was inoculated into 9-day-old chicken embryos and virus was harvested, purified and suspended in 0.9% saline as JEV antigen. The control antigen was prepared by the same method as for the antigen. In the ELISA, the optimal concentrations of antigen coated and dilution factor were selected using 72 test. Ninety-two swine sera negative to haemagglutination inhibition (HI) were tested by this assay and the positive threshold was determined. The results of this study indicate that indirect ELISA has high specificity, sensitivity and reproducability. Simultaneous testing of 74 serum samples from nine pig farms was carried out to compare the existing HI test and the indirect ELISA. The coincidence rate of the two assays was 85.1% (63/74) and no significant difference was observed between them (p > 0.05). This ELISA test can detect 46 swine serum samples qualitatively and the titre of eight swine serum samples through endpoint dilution quantitatively within one 96-well plate.
引用
收藏
页码:159 / 169
页数:11
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