Random segment deletion excision system in based on IS31831 and Cre/loxP Corynebacterium glutamicum

被引：23

作者：

Tsuge, Yota

Suzuki, Nobuaki

Inui, Masayuki

Yukawa, Hideaki

机构：

[1] RITE, Kyoto 6190292, Japan

[2] Nara Inst Sci & Technol, Grad Sch Biol Sci, Nara 6300192, Japan

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2007年 / 74卷 / 06期

关键词：

Corynebacteritun glutamicum; Cre/loxP; IS element; genomic engineering; random genome deletion;

D O I：

10.1007/s00253-006-0788-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A simple and random genome deletion method combining insertion sequence (IS) element IS31831 and the Cre/loxP excision system generated 42 Corynebacterium glutamicum mutants (0.2-186 kb). A total of 393.6 kb (11.9% of C. glutamicum R genome) coding for 331 genes was confirmed to be nonessential under standard laboratory conditions. The deletion strains, generated using only two vectors, varied not only in their lengths but also the location of the deletion along the C. glutamicum R genome. By comparing and analyzing the generated deletion strains, identification of nonessential genes, the roles of genes of hitherto unknown function, and gene-gene interactions can be easily and efficiently determined.

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收藏

页码：1333 / 1341

页数：9

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