Separation of monoclonal antibodies from cell-culture supernatants and ascites fluid using thiophilic agarose

被引：5
|
作者：
Bog-Hansen, TC [1 ]
机构：
[1] Univ Copenhagen, Panum Inst, Prot Lab, Copenhagen, Denmark
来源：
MOLECULAR BIOTECHNOLOGY | 1997年 / 8卷 / 03期
关键词：
thiophilic adsorption; one-step purification of antibodies; affinity chromatography;
D O I：
10.1007/BF02760781
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A one-step purification procedure will yield monoclonal antibodies from cell-culture supernatants and ascites fluids. The chromatographic adsorbant is thiophilic argose, i.e., beaded agarose gel coupled with ligands of thiophilic nature, often with a sulfone group and a sulfur atom. The chromatographic procedure is simply adsorption, wash, elution. The procedure is simple, efficient, and inexpensive.
引用
收藏
页码：279 / 281
页数：3
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