Effects of galectin-1 on immunomodulatory properties of human monocyte-derived dendritic cells

被引：7

作者：

Dzopalic, Tanja ^{[1
]}

Kostic, Milos ^{[1
]}

Kostic, Milena ^{[2
]}

Marjanovic, Goran ^{[1
,3
]}

Guzina, Jelena ^{[2
]}

Jurisic, Vladimir ^{[4
]}

Nedeljkovic, Biljana Bozic ^{[2
]}

机构：

[1] Univ Nis, Med Fac, Dept Immunol, Nish, Serbia

[2] Univ Belgrade, Fac Biol, Inst Physiol & Biochem Ivan Djaja, Belgrade, Serbia

[3] Clin Ctr Nis, Clin Hematol & Clin Immunol, Nish, Serbia

[4] Univ Kragujevac, Fac Med Sci, Svetozara Markovica 69, Kragujevac 34000, Serbia

来源：

GROWTH FACTORS | 2020年 / 38卷 / 5-6期

关键词：

Galectin-1; immunomodulation; monocyte-derived dendritic cells; T-CELLS; IMMUNE-RESPONSES; ACTIVATION; BINDING; IL-10; INTERLEUKIN-2; CD83; LOCALIZATION; EXPRESSION; RECEPTORS;

D O I：

10.1080/08977194.2021.1947267

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Our study aimed to evaluate the effects of Gal-1 in dose depending manner on maturation and immunomodulatory properties of monocyte-derived (Mo) DCs in-vitro. The effects were analyzed by monitoring their phenotypic characteristics, cytokine profile, and the ability to direct the immune response in the co-culture with allogeneic CD4(+)T cells. Gal-1 reduced the expression of CD80 and CD86 molecules on MoDCs compared to untreated MoDCs. Gal-1 at concentrations of 1 and 6 mu g/mL significantly reduced IL-12 production, while the concentration of 3 mu g/mL led to its significant increase. Gal-1 in all concentrations induced a significant increase in the production of IL-10. Treatment of MoDCs with 3 and 6 mu g/mL of Gal-1 stimulated the production of IL-2 and IFN-gamma in the co-culture with CD4(+)T lymphocytes. This study demonstrated a dual immunomodulatory effect of Gal-1 on MoDCs in terms of immune stimulation and immune suppression, depending on the applied concentration.

引用

页码：235 / 246

页数：12

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