Engineering Translation Components for Genetic Code Expansion

被引：15
作者：
Kim, Sooin [1 ]
Yi, Hanbin [1 ]
Kim, Yurie T. [1 ]
Lee, Hyun Soo [1 ]
机构：
[1] Sogang Univ, Dept Chem, 35 Baekbeomro Mapogu, Seoul 04107, South Korea
来源：
JOURNAL OF MOLECULAR BIOLOGY | 2022年 / 434卷 / 08期
关键词：
genetic code; ribosomes; tRNA/aminoacyl-tRNA synthetase pair; codons; elongation factor Tu; TRANSFER-RNA SYNTHETASE; UNNATURAL AMINO-ACIDS; ELONGATION-FACTOR TU; IN-VIVO INCORPORATION; ESCHERICHIA-COLI; SEMISYNTHETIC ORGANISM; PROTEIN-SYNTHESIS; MAMMALIAN-CELLS; MESSENGER-RNA; ORTHOGONAL TRANSLATION;
D O I：
10.1016/j.jmb.2021.167302
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The expansion of the genetic code consisting of four bases and 20 amino acids into diverse building blocks has been an exciting topic in synthetic biology. Many biochemical components are involved in gene expression; therefore, adding a new component to the genetic code requires engineering many other components that interact with it. Genetic code expansion has advanced significantly for the last two decades with the engineering of several components involved in protein synthesis. These components include tRNA/aminoacyl-tRNA synthetase, new codons, ribosomes, and elongation factor Tu. In addition, biosynthesis and enhanced uptake of non-canonical amino acids have been attempted and have made meaningful progress. This review discusses the efforts to engineer these translation components, to improve the genetic code expansion technology. (C) 2021 Elsevier Ltd. All rights reserved.
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页数：17
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