Identification of trifluoperazine metabolites using HPLC-NMR of test mixtures

被引：0

作者：

Dachtler, M

Händel, H

Glaser, T

Lindquist, D

Hawk, RM

Karson, CN

Komoroski, RA

Albert, K

机构：

[1] Univ Tubingen, Inst Organ Chem, D-72076 Tubingen, Germany

[2] Univ Arkansas, Dept Appl Sci, Little Rock, AR 72204 USA

[3] Cent Arkansas Vet Healthcare Syst, Little Rock, AR 72205 USA

[4] Univ Arkansas Med Sci, Dept Radiol, Little Rock, AR 72205 USA

[5] Univ Arkansas Med Sci, Dept Pathol, Little Rock, AR 72205 USA

[6] Univ Arkansas Med Sci, Dept Psychiat, Little Rock, AR 72205 USA

[7] Univ Arkansas Med Sci, Dept Biochem, Little Rock, AR 72205 USA

来源：

MAGNETIC RESONANCE IN CHEMISTRY | 2000年 / 38卷 / 11期

关键词：

NMR; H-1; HPLC-NMR; metabolism; trifluoperazine;

D O I：

10.1002/1097-458X(200011)38:11<951::AID-MRC762>3.0.CO;2-6

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The metabolites of the antipsychotic drug trifluoperazine (TFP) were investigated with on-line coupling of high-performance liquid chromatography (HPLC) and NMR spectroscopy. A chromatographic method was developed using a reversed-phase C-30 silica gel column. Rats were given a cumulative dose of 90 mg kg(-1) TFP dihydrochloride and samples of brain and muscle tissues were extracted after 29 h. The peaks obtained on chromatographic separation were assigned to trifluoperazine and its metabolites. With the help of chromatograms obtained from test mixtures, 1D and 2D HPLC-NMR were used to identify the peaks found for these mixtures. Copyright (C) 2000 John Wiley & Sons, Ltd.

引用

页码：951 / 956

页数：6

共 28 条

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