Surface-Layer Protein from Lactobacillus acidophilus NCFM Inhibits Lipopolysaccharide-Induced Inflammation through MAPK and NF-κB Signaling Pathways in RAW264.7 Cells

被引：57
|
作者：
Wang, Huifang [1 ]
Zhang, Li [2 ]
Xu, Shichen [1 ,2 ]
Pan, Jie [1 ]
Zhang, Qiuxiang [1 ]
Lu, Rongrong [1 ]
机构：
[1] Jiangnan Univ, Sch Food Sci & Technol, 1800 Lihu Ave, Wuxi 214122, Jiangsu, Peoples R China
[2] Minist Hlth, Key Lab Nucl Med, Jiangsu Inst Nucl Med, 20 Qian Rong, Wuxi 214063, Jiangsu, Peoples R China
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2018年 / 66卷 / 29期
基金：
中国国家自然科学基金;
关键词：
Lactobacillus acidophilus NCFM; surface-layer protein; anti-inflammatory; MAPK; NF-kappa B; HYDROGEN-PEROXIDE; MACROPHAGE; PURIFICATION; EXPRESSION; PROBIOTICS; APOPTOSIS; FRACTION; CHEESE;
D O I：
10.1021/acs.jafc.8b02012
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
The objective of our research was to evaluate the molecular mechanism of the anti-inflammatory effects of surface-layer protein (Slp) derived from Lactobacillus acidophilus NCFM in lipopolysaccharide-induced RAW264.7 cells. Our results presented that Sip, with an apparent size of 46 kDa, attenuated the production of TNF-alpha, IL=1 beta, and reactive oxygen species (ROS), by inhibiting the MAPK and NF-kB signaling pathways. In addition, 10 mu g mL(-1) of Slp significantly inhibited NO and PGE(2) production (P < 0.001) through downregulating the expression levels of iNOS and COX-2 protein. Furthermore, Slp was found to inhibit NF-kB p65 translocation into the nucleus to activate inflammatory gene transcription. These findings suggest that Slp is a potential immune-modulating bioactive protein derived from probiotics and holds promise for use as an additive in functional foods.
引用
收藏
页码：7655 / 7662
页数：8
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