Protective effect of Sika Deer bone polypeptide extract on dexamethasone-induced osteoporosis in rats

被引：9
作者：
An, Liping [1 ]
Shi, Liqiang [1 ]
Ye, Yujie [1 ]
Wu, Dankai [2 ]
Ren, Guangkai [2 ]
Han, Xiao [1 ]
Xu, Guangyu [1 ]
Yuan, Guangxin [1 ]
Du, Peige [1 ]
机构：
[1] Beihua Univ, Coll Pharm, Jilin 132013, Jilin, Peoples R China
[2] Jilin Univ Second Hosp, Orthoped Hosp, Dept Orthoped, Changchun 130041, Jilin, Peoples R China
来源：
ELECTRONIC JOURNAL OF BIOTECHNOLOGY | 2021年 / 52卷
关键词：
Bone; Cervus nippon; Dexamethasone; eNOS; Glucocorticoid-induced osteoporosis; Glucocorticoids; Osteoblasts; Osteoporosis; Protective effect; Rats; Sika Deer bone polypeptide extract; GLUCOCORTICOID-INDUCED OSTEOPOROSIS; NITRIC-OXIDE SYNTHASE; ASSOCIATION; OSTEOBLASTS; RISK; GENE;
D O I：
10.1016/j.ejbt.2021.04.002
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Background: Osteoporosis attacks approximately 10% of the population worldwide. Sika Deer (Cervus nippon), one of China's precious traditional medicinal animals, has been widely recorded in ancient Chinese medical books and claimed for centuries to have numerous medical benefits including bone strengthening. This study aimed to find the use of Sika Deer bone in treating osteoporosis according to traditional records and to investigate the protective effect of Sika Deer bone polypeptide extract on glucocorticoid-induced osteoporosis (GIOP) in rats. Results: Sika Deer bone polypeptide extract could increase serum Ca2+ and BGP, decrease serum P3+, ALP, PTH, and CT, but had no effect on serum NO in rats with GIOP. The immunohistochemical iNOS results of the rats' distal femur were negative in each group. Besides the model group, the eNOS color reaction in osteoblasts was strongly positive in the other three groups. Conclusions: Sika Deer bone polypeptide extract can improve pathological changes in the microstructure and stimulate the expression of eNOS in osteoblasts. The protective effect on bone might be mediated by eNOS-dependent NO generation. (C) 2021 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V.
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页码：52 / 58
页数：7
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