Variation of female preference for male coloration in the eastern mosquitofish Gambusia holbrooki

The preference for melanistic males was studied in two populations of eastern mosquitofish (Gambusia holbrooki, Pisces: Poeciliidae), one from Florida and one from northern Italy. Melanism in the eastern mosquitofish is a Y-linked character, expressed in males only. Melanistic males have black spots varying in size and number. In the Florida population, melanistic males are common, whereas in the Italian population they have never been observed. Females were male-deprived for at least 2 months before being tested in a dichotomous choice chamber. Italian females showed a significant preference for unpigmented males from their own population, whereas Florida females preferred melanistic males. When given the choice between males with few (<10% of the body surface) and males with many (>50%) black spots, Italian females preferred males with few black spots and Florida females those with many black spots. The preference of the Italian females for unpigmented males was confirmed in females reared from birth to maturity in the presence of only melanistic males. The preference of Florida females for melanistic males was also confirmed in females reared from birth to maturity in the presence of only unpigmented males. Altogether, these results demonstrate that in the eastern mosquitofish there is polymorphism in female preference and that this preference does not have an environmental basis.