Peculiarities of Interaction of Porphyrins with tRNA at Low Ionic Strength

被引：5

作者：

Dalyan, Y. ^{[1
]}

Vardanyan, I. ^{[1
]}

Chavushyan, A. ^{[1
]}

Balayan, G. ^{[2
]}

机构：

[1] Yerevan State Univ, Fac Phys, Dept Mol Phys, Yerevan 375049, Armenia

[2] AI Mndzhoyan Fine Organ Chem Inst, Yerevan 0014, Armenia

来源：

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS | 2010年 / 28卷 / 01期

关键词：

ANTITUMOR AGENTS; DNA-BINDING; COMPLEXES; THERMODYNAMICS; STABILIZATION; HOECHST-33258; DERIVATIVES; INHIBITION;

D O I：

10.1080/07391102.2010.10507348

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of meso-tetra-(4N-oxyethylpyridyl)porphyrin (TOEPyP4) and its Zn(II)-, Cu(II)-, Mn(III)-derivatives with tRNA from E.Coli at low ionic strength (mu=0.02M) was studied using UV/Vis spectrophotometry and Circular Dichroism (CD) methods. An unusual Induced Circular Dichroism (ICD) spectra profile of the ZnTOEPyP4-tRNA complex is found. It is demonstrated that ZnTOEPyP4 is ordered in a stack, not only on helical sites, but also on loops of a hairpin form of tRNA. TOEPyP4 and CuTOEPyP4 are able to intercalate in the helical sites of this form of tRNA. MnTOEPyP4 interacts with tRNA via external non-ordered mechanism. It is established that all porphyrins are bound with tRNA more strongly than with DNA.

引用

页码：123 / 131

页数：9

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