Low-cost luminescent scaffolds-based on thiol chitosans by microwave radiation for vertebral disc repair/theragnostic

被引：11

作者：

Borsagli, Fernanda G. L. Medeiros ^{[1
]}

Rodrigues, Jordane S. ^{[1
]}

Aguiar, Rafaella A. ^{[1
]}

Paiva, Aislan Esmeraldo ^{[2
]}

Vasquez, Jhonattan Frank Baez ^{[2
]}

Ramos, Welyson Tiano do Santos ^{[1
]}

Allibrandini, Paulo ^{[1
]}

Rocha, Elem Patricia Alves ^{[1
]}

Goncalves, Max P. ^{[1
]}

de Souza, Fidel Edson ^{[1
]}

机构：

[1] Univ Fed Vales Jequitinhonha & Mucuri UFVJM, Inst Engn Sci & Technol, Ave 01,4050 Cidade Univ, BR-39440039 Janauba, MG, Brazil

[2] Trinity Coll Dublin, AMBER Res Ctr, Sch Chem, Dublin, Ireland

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2022年 / 209卷

关键词：

Thiomers; Mimetic biomaterials; Vertebral disc; Fast diagnosis; Eco-friendly; INTERVERTEBRAL DISC; COMPLEXATION; BIOMATERIALS;

D O I：

10.1016/j.ijbiomac.2022.04.191

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study introduces a new 3D scaffold based on thiolated chitosans with luminescence by microwave radiation using cysteine (Chi_CT_Cys) and 11-mercaptoundecanoic acid (Chi_CT_MUA) for vertebral disc regeneration/theragnostic. These scaffolds were characterized by Raman, PL spectroscopy, swelling, gel-fraction, and morphologies. Cytocompatibility and mechanical behavior were evaluated. Raman showed that disulfide bonds improved the grafting degree (Chi_CT_Cys (1072 +/- 136) mu mol.g(-1) and Chi_CT_MUA (3245 +/- 105) mu mol.g(-1)). Morphologies showed interesting characteristics. Swelling behavior showed that Chi_CT_MUA presented a slight minor swelling (2101 +/- 251) % compared to Chi_CT_Cys (2589 +/- 188) %. Differently, gel-fraction showed that the chemical stability of Chi_CT_Cys was worse (29 +/- 4) % than Chi_CT_MUA (15 +/- 3) %. PL showed a possibility to use theragnostic evaluation of points of greater compression in a vertebral disc. The mechanical behavior of Chi_CT_MUA presented better results ((70 +/- 3) MPa) than Chi_CT_Cys ((37 +/- 3) MPa). Cytocompatible showed that the scaffolds presented cell viability >90%. Thusly, these 3D scaffolds presented an incredible potential for tissue engineering applications.

引用

页码：2109 / 2118

页数：10

共 37 条

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